关键词: 虫生真菌, 共培养, 石蒜绵粉蚧, 室内侵染活性, 室内防治效果

Abstract: To evaluate the biocontrol potential and prospect of Metarhizium anisopliae FM-03 and Lecanicillium lecanii LL-01 co-culture against the quarantine pest Phenacoccus solani, the affinity between these two strains, the sporulation and biomass of co-culture strains, and their laboratory pathogenicity and control efficiency against the mealybug were determined in this study. Results showed that these two strains grew normally and there was no obvious antagonistic effect to each other when they were co-cultured, because the affinity between these two strains was strong. The substance accumulation and insecticidal activity of co-culture strains FM-03 and LL-01 at the inoculation ratios 2:3 were optimal and significantly higher than the single culture strain. The total sporulation and biomass after ten days’ co-culture were 5.53×10¹⁰conidia/cm²and 0.72 g, respectively, and the sporulation proportions of strains FM-03 and LL-01 were 49.34% and 50.66%, respectively. The LC₅₀ value against the mealybug at ten day infection was 2.84×10⁴conidia/cm². The cumulative mortality, co-infection proportion and LT₅₀ values for a dose of 1×10⁸ conidia/mL were 97.73%, 67.01% and 3.63 d, respectively. In addition, the laboratory contro1 efficiency and co-infection proportion of the co-culture strains also enhanced with time, reaching 86.14% and 60.17% at twelve days post treatment with 1×10⁸ conidia/mL. In conclusion, strains FM-03 and LL-01 co-culture at the suitable inoculation proportions has significant synergistic effect on the control of P. solani, suggesting that these two strains have an excellent potential in the development of compound biocontrol agent.

Key words: entomogenous fungus, co-culture, Phenacoccus solani, laboratory pathogenicity, laboratory control efficiency