性诱剂与生防真菌联用防治大豆食心虫

doi:10.16409/j.cnki.2095-039x.2025.07.006

摘要/Abstract

摘要： 为探索哈尔滨地区田间大豆食心虫的生物防治措施，本文开展了性诱剂与生防真菌联用对田间大豆食心虫的防控效果研究。2023年单项生防措施研究结果表明，诱芯A和金龟子绿僵菌CQMa421比诱芯B和球孢白僵菌ZJU435更适合在哈尔滨地区防治田间大豆食心虫。2024年联合防治研究结果表明，在大豆食心虫羽化前放置60个诱芯A/hm²处理的虫荚率、虫食率和为害株率防效均在36.17%以下，金龟子绿僵菌CQMa421低推荐剂量600 mL/hm²和高推荐剂量1200 mL/hm²在8月11日单独施用对田间大豆食心虫的虫荚率、虫食率和为害株率均显著低于氯虫苯甲酰胺75 g/hm²处理； 60个诱芯A/hm²与1200 mL/hm²金龟子绿僵菌CQMa421联合施用与氯虫苯甲酰胺处理的虫荚率、虫食率、为害株率和产量无显著差异，防效均达到78%以上。综上所述，性诱剂与金龟子绿僵菌联用具有良好的防治效果，可以作为哈尔滨地区田间大豆食心虫的生物防控措施。

关键词: 大豆食心虫, 性诱剂, 生防真菌, 田间防效, 绿色防控

Abstract: This study aims to explore the biological control measures of Leguminivora glycinivorella in Harbin by combining sex pheromone with biocontrol fungus in field tests. In 2023, the results of individual biocontrol measure tests suggested that lure A and Metarhizium anisopliae CQMa421 were more suitable than lure B and Beauveria basiana ZJU435, respectively, for controlling L. glycinivorella in Harbin. In 2024, the test results of sex pheromone combined with M. anisopliae revealed that the pod damage rate, seed damage rate and plant damage rate were all less than 36.17% when the sex pheromone traps of lure A were setup at 60 per hm² before the borer adult emergence. The pod damage rate, seed damage rate and plant damage rate were significantly lower when the plants were sprayed with the low (600 mL/hm²) and high (1200 mL/hm²) recommended dosage of M. anisopliae CQMa421 on August 11 than when 75 g/hm² of chlorantraniliprole was sprayed. There was no significant difference in pod damage rate, seed damage rate and plant damage rate and yield between the sex pheromone (lure A) combined with 1200 mL per hm² of M. anisopliae CQMa421 and chlorantraniliprole, all being>78%. In summary, sex pheromone combined with M. anisopliae is prospective as a biological control measure for L. glycinivorella in Harbin.

Key words: Leguminivora glycinivorella, sex pheromone, biocontrol fungus, field control efficiency, green prevention and control

中图分类号:

S476.2

王宇, 王克勤, 王晓曦, 刘兴龙, 王春. 性诱剂与生防真菌联用防治大豆食心虫[J]. 中国生物防治学报, 2026, 42(1): 191-197.

WANG Yu, WANG Keqin, WANG Xiaoxi, LIU Xinglong, WANG Chun. Sex Pheromone Combined with Biocontrol Fungus for Control of Leguminivora glycinivorella[J]. Chinese Journal of Biological Control, 2026, 42(1): 191-197.

参考文献

[1] 任振涛, 赵海盟, 周宜君, 等. 大豆食心虫越冬种群解除滞育规律研究[J]. 应用昆虫学报, 2023, 60(6): 1777-1784.
[2] 李旋, 崔娟, 秦昊东, 等. 大豆食心虫田间性诱监测与防治指标研究[J]. 中国油料作物学报, 2020, 42(4): 701-707.
[3] 王克勤, 李新民, 刘春来, 等. 黑龙江省大豆品种对大豆食心虫抗性评价[J]. 大豆科学, 2006(2):153-157.
[4] 兰英, 王泽胤, 赵秀梅, 等. 4种赤眼蜂对大豆食心虫的防治效果[J]. 中国植保导刊, 2023, 43(12): 62-66.
[5] 付晓霞, 徐伟, 卢爱军, 等. 大豆植株挥发物组分变化与大豆食心虫为害的关系[J]. 吉林农业大学学报, 2014, 36(4): 389-394.
[6] 徐伟, 张益恺, 董亚南, 等. 不同品种大豆蛋白酶抑制剂活性及其对大豆食心虫幼虫的影响[J]. 吉林农业大学学报, 2019, 41(3): 267-273.
[7] 赵宇, 刘佳, 路杨, 等. 螟黄赤眼蜂携带球孢白僵菌防治大豆食心虫[J]. 中国生物防治学报, 2023, 39(4): 760-766.
[8] 李启云, 路杨, 隋丽, 等. 植物保护与农业绿色发展[J]. 吉林农业大学学报, 2021, 43(1): 9-15.
[9] 王宇, 王克勤, 王晓曦, 等. 哈尔滨地区不同亚洲玉米螟性诱剂诱捕效果研究[J]. 植物保护, 2023, 49(6): 338-342, 349.
[10] 车晋英, 陈华, 陈永明, 等. 4种不同性诱剂对玉米草地贪夜蛾诱集作用[J]. 植物保护, 2020, 46(2): 261-266.
[11] 刘伟, 杨鹏, 蒋龙, 等. 亚洲玉米螟性信息素合成研究进展[J]. 安徽农业科学, 2013, 41(20): 8531-8535.
[12] 刘万才, 刘振东, 朱晓明, 等. 我国昆虫性信息素技术的研发与应用进展[J]. 中国生物防治学报, 2022, 38(4): 803-811.
[13] 彭国雄, 张淑玲, 张维, 等. 杀虫真菌与苏云金芽胞杆菌对草地贪夜蛾的联合室内杀虫活性研究[J]. 中国生物防治学报, 2019, 35(5): 735-740.
[14] Faria M R D, Wraight S P. Mycoinsecticides and Mycoacaricides: A comprehensive list with worldwide coverage and international classification of formulation types[J]. Biological Control, 2007, 43(3): 237-256.
[15] 赵钰, 毛瑞霞, 邢培翔, 等. 爪哇虫草菌与巴氏新小绥螨组合防控二斑叶螨技术初探[J]. 中国生物防治学报, 2024, 40(6): 1250-1259.
[16] 王红, 徐忠新, 韩岚岚, 等. 基于线粒体COI基因序列的大豆食心虫中国东北地理种群遗传多样性分析[J]. 昆虫学报, 2014, 57(9): 1051-1060.
[17] 程媛, 韩岚岚, 于洪春, 等. 性诱剂、赤眼蜂和化学药剂协同防治大豆食心虫的研究[J]. 应用昆虫学报, 2016, 53(4): 752-758.
[18] Clarkson J M, Charnley A K. New insights into the mechanisms of fungal pathogenesis in insects[J]. Trends Microbiology, 1996, 4(5): 197-203.
[19] 胡丰林, 樊美珍, 李增智, 等. 一种白僵菌代谢产物中生物活性物质的研究Ⅰ 具有清除自由基的活性物质的分离和制备. [J]. 菌物系统, 2000(4): 522-528.
[20] Kershaw M J, Moorhouse E R, Bateman R, et al. The role of destruxins in the pathogenicity of Metarhizium anisopliae for three species of insects[J]. Journal of Invertebrate Pathology, 1999, 74: 213-223.
[21] 张礼生, 张泽华, 高松, 等. 绿僵菌生物农药的研制与应用[J]. 中国生物防治, 2006(S1): 141-146.
[22] 张亚倩, 谷静秀, 苑士涛, 等. 2亿活孢子/g金龟子绿僵菌CQMa421颗粒剂防治花生地老虎田间药效试验[J]. 农药, 2019, 58(9): 684-686.
[23] 张秀霞, 赵忠范, 毛晓红, 等. 金龟子绿僵菌CQMa421与 3种杀虫剂对瓜蚜的联合毒力[J]. 农药, 2020, 59(1): 74-78.
[24] 车正明, 朱楚波, 李正祥, 等. 金龟子绿僵菌CQMa 421防治水稻白背飞虱试验[J]. 云南农业科技, 2018(6): 42-43.
[25] 李君保, 黎萍, 冉文浩, 等. 绿僵菌CQMa421对水稻稻飞虱和稻纵卷叶螟的田间防效初探[J]. 南方农业, 2019, 13(10): 37-39.
[26] 王立平, 孙丽娜, 崔晓蕊. 金龟子绿僵菌可分散油悬浮剂防治水稻二化螟试验[J]. 农业与技术, 2019, 39(16): 40-42.
[27] 卓富彦, 周若男, 蒙玲玲, 等. 金龟子绿僵菌CQMa421对棉蚜、绿盲蝽的田间防效[J]. 植物保护, 2024, 50(3): 350-354, 360.