1.2%白屈菜红碱·0.8%蛇床子素悬浮剂的研制与应用

doi:10.16409/j.cnki.2095-039x.2025.02.070

摘要/Abstract

摘要： 近年来，稻曲病已成为水稻主要的真菌病害之一，不但影响水稻产量和稻米品质，其病原菌还会产生毒素，严重影响食品安全。利用植物源农药进行合理复配可提高其防治效果并有效缓解抗药性的产生。本研究中单剂抑菌试验显示白屈菜红碱的抑制中浓度EC₅₀是1.17 μg/mL，蛇床子素的EC₅₀是9.28 μg/mL，白屈菜红碱:蛇床子素在6:4时，药剂浓度为7 μg/mL时抑菌率为81.86%，EC₅₀是0.02 μg/mL，增效系数（coefficient of synergism，SR）是100.49（SR>1.5即为增效作用）（共毒系数显示两种药剂复配仅表现为相加作用）。筛选出的白屈菜红碱复配蛇床子素悬浮剂配方，质控指标合格，粒径分布总体比较集中（D₉₀=9.683 μm），在水中分散比较均匀。制备的复配药剂生物防治对稻曲病的田间防治效果较好，在稀释100倍液时防治效果可达76.36%。植物源成分环保、高效，病原菌不易产生抗药性，保证人畜健康，具有深远意义及巨大的市场潜力。

关键词: 白屈菜红碱, 蛇床子素, 悬浮剂配方, 稻曲病, 防治效果

Abstract: Recently, rice false smut (RFS) has emerged as a main fungal disease affecting rice. It not only impacts the crop yield and quality but also produces toxins that pose a serious threat to food safety. The judicious blending of botanical fungicides can enhance their efficacy and effectively mitigate the development of fungicide resistance. In our study, single-agent antibacterial tests showed that the EC₅₀ value for chelerythrine was 1.17 μg/mL, while 9.28 μg/mL for osthole. When combined at a mass ratio of 6:4, the mixture exhibited an 81.86% inhibition rate at 7 μg/mL, with an EC₅₀ value of 0.02 μg/mL and a synergistic ratio (SR) of 100.49. The selected formulation of chelerythrine mixed with osthole met the quality control standards, exhibiting a generally concentrated particle size distribution (D₉₀=9.683 μm) and dispersing evenly in water. The formulation of chelerythrine mixed with osthole in a suspension preparation demonstrated good biological control effect against RFS, with efficacy of 76.36% at a 100-fold dilution Due to their environmental friendliness, high efficacy, low risk of pathogen resistance and safety to human and livestock, the botanical fungicides have the great potential for further development and application.

Key words: chelerythrine, osthole, suspension concentrate formulation, rice false smut, control effect

中图分类号:

S476

韦庆慧, 宋伟丰, 师正浩, 李志勇, 潘亚清, 王芳媛, 张颖, 刘凯. 1.2%白屈菜红碱·0.8%蛇床子素悬浮剂的研制与应用[J]. 中国生物防治学报, 2025, 41(6): 1412-1420.

WEI Qinghui, SONG Weifeng, SHI Zhenghao, LI Zhiyong, PAN Yaqing, WANG Fangyuan, ZHANG Ying, LIU Kai. Preparation and Application of Suspension of 1.2% Chelerythrine Mixed with 0.8% Osthole[J]. Chinese Journal of Biological Control, 2025, 41(6): 1412-1420.

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