东湖假单胞ZF510可湿性粉剂配方优化及其对白菜软腐病的防治效果

doi:10.16409/j.cnki.2095-039x.2025.02.060

摘要/Abstract

摘要： 为获得防治白菜软腐病的高效生防菌剂，本研究采用单因素试验和正交试验设计优化东湖假单胞菌ZF510发酵工艺，筛选适宜载体、助剂，制备可湿性粉剂，并对其稳定性及盆栽防效进行评价。结果表明，最适发酵培养基为糊精25 g/L，酵母粉5 g/L，NaCl 1.5 g/L，MgSO₄·7H₂O 1.5 g/L；最佳发酵条件为温度30℃，pH值7.5，接种量2%(v/v)，装液量100 mL/250 mL，转速200 r/min；可湿性粉剂最佳配方载体为白炭黑，润湿剂1.5% Morwet EFW，分散剂3% Morwet IP，紫外保护剂0.5%羧甲基纤维素以及稳定剂1.5%羧甲基纤维素钠；所得制剂活菌数为7.9×10¹⁰ CFU/g，润湿时间为103 s、悬浮率为80.58%，pH为7.82，含水量为2.11%，杂菌率为2.94%，97.02%能通过74 μm标准筛，符合国家标准，质量合格。盆栽试验结果表明，东湖假单胞菌ZF510可湿性粉剂500倍液处理能有效防治白菜软腐病，防治效果达67.92%。本研究为东湖假单胞菌ZF510可湿性粉剂的研发与应用奠定了技术基础。

关键词: 东湖假单胞菌ZF510, 白菜软腐病, 发酵条件优化, 可湿性粉剂, 防治效果

Abstract: To obtain an efficient biocontrol antimicrobial agent against Chinese cabbage soft rot, single factor test and orthogonal test design were used to optimize the fermentation process of Pseudomonas donghuensis ZF510. Subsequently, the appropriate carriers and adjuvants were screened to prepare a wettable powder, and its stability as well as pot control efficacy were evaluated. The results showed that the optimal fermentation medium for strain ZF510 was dextrin 25 g/L, yeast powder 5 g/L, NaCl 1.5 g/L, MgSO₄·7H₂O 1.5 g/L. The optimal fermentation conditions were as follows: fermentation temperature 30 ℃ , pH 8.0, inoculation concentration 2%, 100 mL medium in 250 mL flask, rotation speed 200 rpm. The optimal formulation of WP was white carbon black as carrier, 1.5% Morwet EFW wetting agent, 3% Morwet IP dispersing agent, 0.5% carboxymethyl cellulose as protective agent, 1.5% sodium carboxymethyl cellulose as stabilizer. The viable count of the preparation was 7.9×10¹⁰ CFU/g, wetting time 103 s, suspension rate 80.58%, pH 7.82, the moisture content was 2.11%, impurity rate 2.94%, and the ratio transiting standard test sieve with 74 μm was 97.02%, which was in line with national standards and quality standards. Pot experiment demonstrated the 500-fold wettable powder of Pseudomonas donghuensis ZF510 has a good control effect on Chinese cabbage soft rot, with a control efficacy of 67.92%. This study lays the technical foundation for the development and application of Pseudomonas donghuensis ZF510 wettable powder.

Key words: Pseudomonas donghuensis ZF510, Chinese cabbage soft rot, optimization of fermentation condition, wettable powder, control effect

中图分类号:

S476

刘阳, 覃诗扬, 石彬, 谢学文, 石延霞, 柴阿丽, 范腾飞, 李宝聚, 李磊. 东湖假单胞ZF510可湿性粉剂配方优化及其对白菜软腐病的防治效果[J]. 中国生物防治学报, 2025, 41(5): 1119-1132.

LIU Yang, QIN Shiyang, SHI Bin, XIE Xuewen, SHI Yanxia, CHAI Ali, FAN Tengfei, LI Baoju, LI Lei. Formulation Optimization of Pseudomonas donghuensis ZF510 Wettable Powder and Its Control Effect on Soft Rot of Chinese Cabbage[J]. Chinese Journal of Biological Control, 2025, 41(5): 1119-1132.

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