一株侵染斜纹夜蛾的核型多角体病毒的分离鉴定及毒力分析

doi:10.16409/j.cnki.2095-039x.2025.09.014

摘要/Abstract

摘要： 从云南省大理州湾桥镇烟田采集的自然罹病死亡的疑似被病毒感染的斜纹夜蛾幼虫，对体内病原物进行富集分离，通过形态学观察、分子鉴定及系统发育分析对该病原种类进行了鉴定，并采用含有病毒的人工饲料饲喂法生物测定了该病原物在斜纹夜蛾体内的复制特征和对3龄斜纹夜蛾幼虫的毒力。结果表明，罹病斜纹夜蛾幼虫体的病原为斜纹夜蛾核型多角体病毒（Spodoptera litura Nucleopolyhedrovirus），编号为SpltMNPV-DL01。该病毒毒株可在斜纹夜蛾幼虫体内迅速复制，当1.0×10⁶ PIB/mL病毒饲喂斜纹夜蛾3龄幼虫5 d后，体内SpltMNPV-DL01基因组拷贝数多达8.85×10⁶拷贝数/μL。用1.0×10⁴、1.0×10⁵、1.0×10⁶、1.0×10⁷和1.0×10⁸ PIB/mL核型多角体病毒悬浮液饲喂斜纹夜蛾3龄幼虫，LT₅₀分别为7.3 d、7.0 d、6.3 d、5.2 d和4.8 d，毒力回归方程分别为Y＝ -3.452+0.474X、Y＝ -3.551+0.508X、Y＝ -3.314+0.524X、Y＝ -3.043+0.560X和Y＝ -2.722+0.573X，处理后第5、6和7 d的LC₅₀分别是10^7.806、10^6.285和10^4.700 PIB/mL，第8 d时，斜纹夜蛾3龄幼虫的累计死亡率分别为72.22%、75.60%、84.72%、91.67%和98.61%。该研究将丰富斜纹夜蛾核型多角体病毒毒株资源，毒株SpltMNPV-DL01能够有效侵染斜纹夜蛾幼虫并将其致死，可作为优良菌株用于防治斜纹夜蛾生物农药的进一步研发。

关键词: 斜纹夜蛾, 核型多角体病毒, 毒力测定, 生物防治

Abstract: This study enriched and isolated the pathogens from the dead Spodoptera litura larvae collected from the tobacco fields in Wanqiao Town, Dali Prefecture, Yunnan Province. Through morphological observation, molecular identification, and phylogenetic analysis, the pathogen species was identified. The droplet-feeding method was used to determine the replication characteristics of the pathogen in S. litura and its virulence to the 3^rd instar S. litura larvae. The results showed that the pathogen of the diseased S. litura larvae was S. litura nucleopolyhedrovirus, named SpltMNPV-DL01. This virus strain can replicate rapidly in the larvae of S. litura. When the 3^rd instar S. litura larvae were infected with 1.0×10⁶ PIB/mL virus for 5 days, the genomic copy number of SpltMNPV-DL01 in the body was as many as 8.85× 10⁶ copies/μL. When the 3^rd instar S. litura larvae were inoculated with 1.0× 10⁴, 1.0 ×10⁵, 1.0×10⁶, 1.0×10⁷, and 1.0× 10⁸ PIB/mL nucleopolyhedrovirus, the LT₅₀ values were 7.3 d, 7.0 d, 6.3 d, 5.2 d, and 4.8 d, respectively. The corresponding virulence regression equations were Y＝-3.452+0.474X, Y＝ -3.551+0.508X, Y＝ -3.314+0.524X, Y＝ -3.043+0.560X, and Y＝ -2.722+0.573X, respectively. The LC₅₀ on the 5^th, 6^th, and 7^th days of treatment were 10^7.806, 10^6.285, and 10^4.700 PIB/mL, respectively. On the 8^th day, the cumulative mortality rates of the 3^rd instar S. litura larvae were 72.22%, 75.60%, 84.72%, 91.67%, and 98.61%, respectively. The results provide prospective for the application and development of S. litura nucleopolyhedrovirus.

Key words: Spodoptera litura, nuclear polyhedrosis virus, virulence determination, biological control

中图分类号:

S476.1

杨光源, 张轲, 王广, 蔡浩升, 李政宾, 谢永辉, 茶陈文, 顾小飞, 陈斌. 一株侵染斜纹夜蛾的核型多角体病毒的分离鉴定及毒力分析[J]. 中国生物防治学报, 2026, 42(1): 208-217.

YANG Guangyuan, ZHANG Ke, WANG Guang, CAI Haosheng, LI Zhengbin, XIE Yonghui, CHA Chenwen, GU Xiaofei, CHEN Bin. Isolation, Identification and Virulence Analysis of a Nucleopolyhedrovirus Infecting Spodoptera litura[J]. Chinese Journal of Biological Control, 2026, 42(1): 208-217.

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