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Control of Apple Postharvest Gray Mold by Aureobasidium pullulans

GONG Wenfeng1,2, LI Yuefei1, SHANGGUAN Nini1, ZHAO Xinbei1, WANG Yang1, MA Qing1   

  1. 1. State Key Laboratory of Crop Stress Biology for Arid Areas/College of Plant Protection, Northwest A & F University, Yangling 712100, China;
    2. College of Agriculture and Animal Husbandry, Tibet University, Linzhi 860000, China
  • Received:2015-04-21 Online:2016-04-08 Published:2016-04-08

Abstract: A yeast isolate 2SP which had significant control efficiency against Botrytis cinerea was screened out by serial dilution technique and fruit-inoculation test on apple surface. The spore suspension of the isolate exhibited 78.45% of disease control of apple gray mold and 90.0% of spore germination inhibition to B. cinerea. However, the cell free culture filtrate and autoclaved cell cultures had a weak control effect, suggesting that the main action mechanism of the yeast isolate was the competition of nutrient and space. The isolate 2SP could live on apple surface wounds at least one month at 4℃, and the population of the yeasts was higher than that of the beginning. Based on 26S rDNA D1/D2 sequence analysis, the yeast isolate was preliminary identified as Aureobasidium pullulans.

Key words: biocontrol, Botrytis cinerea, postharvest disease, Aureobasidium pullulans

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