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journal1 ›› 2016, Vol. 32 ›› Issue (5): 604-609.DOI: 10.16409/j.cnki.2095-039x.2016.05.009

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Effect of Growth Stages and Nitrogen Fertilization on the Volatiles of Rice Stem Borer Trap Plant Vetiver Grass (Vetiveria zizanioides)

LU Yanhui1, GAO Guangchun2, ZHENG Xusong1, WANG Guorong3, LÜ Zhongxian1   

  1. 1. Institute of Plant Protection and Microbiology of Zhejiang Academy of Agricultural Science, Hangzhou 310021, China;
    2. College of Medicine Science, Jiaxing University, Jiaxing 314001, China;
    3. Xiaoshan Agriculture Bureau, Hangzhou 211203, China
  • Received:2016-03-22 Online:2016-10-08 Published:2016-10-08

Abstract: Volatiles of vetiver grass Vetiveria zizanioides play an important role in trapping female moth of rice stem borer. To clarify the effect of different growth stages and nitrogen fertilization on the volatiles, volatile chemicals were collected from stem leaf of vetiver grasses during three different growth stages and from plants received three different nitrogen fertilization levels by dynamic headspace absorption method, and were analyzed by gas chromatography and mass spectrometry (GC-MS). A total of 23 kinds of compounds were isolated and identified, most of which were alkanes and terpenes chemicals. Among them, the relative content of n-tetradecane in stems and leaves of vetiver in the III growth stage (31.62%) was significantly higher than those in the I and II growth stages, while the relative contents of other detected compounds in different growth stages were not significantly different. The relative contents of α-pinene and nonanal significantly decreased after application of nitrogen fertilizer, while those of cedrol significantly increased after application of nitrogen fertilizer. However, the differences of these three compounds between nitrogen application levels (10 and 20 g/cluster) were not significantly different. Our results indicate that different growth stages and different nitrogen levels have only a slight effect on volatiles of vetiver. These results are of significance in guiding utilization of vetiver to trap rice stem borers.

Key words: growth stages, nitrogen fertilizer, vetiver grass, pant volatiles, trap plant, rice stem borer

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