Obtaining Nonpathogenic Biological Control Strains against Sweetpotato Fusarium Wilt by Agrobacterium-mediated Transformation

doi:10.16409/j.cnki.2095-039x.2016.05.010

Abstract

Abstract: Sweetpotato Fusarium wilt is an important fungal disease caused by Fusarium oxysporum f. sp. batatas. In this study, Agrobacterium-mediated transformation was applied to randomly insert the T-DNA containing GFP gene into the genome of F. oxysporum and 711 mutants were obtained. Nonpathogenic mutant strains 50-5, 55-7 and 103-4 were selected from the mutant library. The sporulation capacity of 50-5 and 103-4 was lower compared with the wild type strain. All the 3 nonpathogenic mutant strains showed antagonistic effect when being co-cultured with the wild-type strain. When the spore concentration of both nonpathogenic mutants and pathogenic strains was 5×10⁵ CFU/mL, the control efficiency of 50-5, 55-7, 103-4 increased with the prolonging pretreatment time and then became stable of 75.65%, 83.80%, 86.95% after 16, 8, 8 h pretreatment, respectively. In conclusion, these three nonpathogenic mutants could effectively control sweetpotato Fusarium wilt and Agrobacterium-mediated transformation could be an effective method to create nonpathogenic biological control agents of other fungal species.

Key words: sweetpotato Fusarium wilt, Fusarium oxysporum, biological control, nonpathogenic strain, Agrobacterium-mediated transformation

CLC Number:

S476

ZHANG Hong, LIN Zhijian, LIN Zhaomiao, LI Guoliang, XU Yongqing, LIU Zhonghua, QIU Yongxiang, LI Huawei, JI Rongchang, LUO Wenbin, TANG Hao, QIU Sixin. Obtaining Nonpathogenic Biological Control Strains against Sweetpotato Fusarium Wilt by Agrobacterium-mediated Transformation[J]. journal1, 2016, 32(5): 610-618.

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