Screening,Identification and Evaluation of Antagonistic Bacteria for the Control of Pear Ring Rot and Anthracnose during Storage

doi:10.16409/j.cnki.2095-039x.2017.01.017

Abstract

Abstract: Pear ring rot caused by Botryosphaeria berengeriana and pear anthracnose caused by Colletotrichum gloeosporioides are two important postharvest diseases and cause significant economic losses. The aim of this study was to screen and evaluate the antagonistic bacteria against both B. berengeriana and C. gloeosporioides. Three hundred and sixty five isolates were isolated and purified from the diseased leaves and pomes, the healthy leaves and pomes, and soil from the orchards of Huaiyin, Huaian and Xuyi, Jiangsu Province. The cultivar of tested pear was Japanese pear cv. hosui, China pear cv. Dangshan and Huangguan. Antagonistic examination against B. berengeriana and C. gloeosporioides in vitro revealed that 4 strains of all tested isolates showed strong and steady antagonism, with the inhibition rate of 73.45%-80.36% against B. berengeriana and 72.02%-76.79% against C. gloeosporioides, respectively. The inoculating experiments on pear pomes showed that strain N106-2 could effectively inhibit necrosis spread of two fungal pathogens, with the inhibition rate of 68.58% on Huangguan pear and 56.98% on Dangshan pear against B. berengeriana, and 56.71% on Huangguan pear against C. gloeosporioides, respectively. The 16S rDNA sequence analysis showed that strains N24-2, N103-1 and N106-2 belong to Bacillus spp. and strain NF22-1 belongs to Pseudomonas spp.

Key words: Botryosphaeria berengeriana, Colletotrichum gloeosporioides, antagonistic bacteria

CLC Number:

S476

LIU Youzhou, LIANG Xuejie, QIAO Junqing, LIU Yongfeng, SHAO Mingcan. Screening,Identification and Evaluation of Antagonistic Bacteria for the Control of Pear Ring Rot and Anthracnose during Storage[J]. journal1, 2017, 33(1): 121-127.

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