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Chinese Journal Of Biological Control ›› 2020, Vol. 36 ›› Issue (2): 220-230.DOI: 10.16409/j.cnki.2095-039x.2020.02.007

• RESEARCH REPORTS • Previous Articles     Next Articles

Identification of Bacillus HN-2 and Analysis of Its Antifungal Properties

WANG Yu, TAN Zheng, WEI Dandan, MIAO Weiguo, LIU Wenbo, JIN Pengfei   

  1. Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests, Ministry of Education/College of Plant Protection, Hainan University, Haikou 570228, China
  • Received:2020-01-22 Online:2020-04-08 Published:2020-04-16

Abstract: The strain HN-2 was isolated from soil. Through morphological, physiological and biochemical characteristics identification, phylogenet tree analysis of 16S rDNA sequence and specific PCR detection, the strain was identified to be Bacillus velezensis. The n-butanol extract, ammonium sulfate precipitation extract and acidification extract of B. velezensis HN-2 demonstrated strong antifungal activity against C. gloeosporioides, with the diameters of inhibition zone of 20.93±0.3 mm, 12.82±0.3mm and 13.57±0.3 mm, respectively. The EC50 value of n-butanol extract was 70.62 μg/mL. Mass spectrum analysis showed that lipopeptide was the main active substance of the strain HN-2, consisting of surfactin, fengycin and iturin. The mycelia of C. gloeosporioides treated with n-butanol extract were distorted, swollen and deformed. Application of n-butanol can reduce the development of C. gloeosporioides on the punctured epidermis of mango. It could be concluded that B. velezensis HN-2 had strong antifungal activity and the main antifungal substance was lipopeptide.

Key words: Bacillus velezensis, lipopeptide, antifungal activity, Colletotrichum gloeosporioides, EC50

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