Screening and Inhibition Effect of Antagonistic Bacteria against Cucumber Anthracnose Caused by Colletotrichum orbiculare

doi:10.16409/j.cnki.2095-039x.2016.02.012

Abstract

Abstract: A bacterial strain WF-3 isolated from rhizosphere soil of cucumber in a continuous cucumber cropping system, exhibited a high antagonistic effect on cucumber anthracnose caused by Colletotrichum orbiculare. This strain was identified as Bacillus methylotrophicus according to its morphological, physiological and biochemical characterizations and the analysis of 16S rDNA gene sequence. In this paper, we have investigated the control effect of WF-3 against cucumber anthracnose via pot and field experiments. Results showed that in the pot experiment the control effect was 66.48%, while in a two-year field test conducted with the cultures of WF-3, the good control effect of 68.14% and 73.70% was obtained in 2013 and 2014, respectively, which proved that WF-3 can effectively control cucumber anthracnose.

Key words: Colletrichum orbiculare, antagonistic bacteria, Bacillus methylotrophicus, cucumber

CLC Number:

S432
S476

XIE Xuewen, DONG Ruili, SHI Yanxia, CHAI Ali, ZHU Fadi, LI Baoju. Screening and Inhibition Effect of Antagonistic Bacteria against Cucumber Anthracnose Caused by Colletotrichum orbiculare[J]. journal1, DOI: 10.16409/j.cnki.2095-039x.2016.02.012.

References

[1] 胡伟, 赵兰凤, 张亮, 等. 香蕉枯萎病生防菌AF11的鉴定及其定殖研究[J]. 中国生物防治学报, 2012, 28(3):387-393.
[2] 黄曦, 张荣灿, 王何健, 等. 枯草芽孢杆菌ON-6菌株抑制荔枝炭疽菌活性物质的初步研究[J]. 中国农学通报, 2011, 27(13):188-193.
[3] 郝卫宁, 李辉, 胡美英, 等. 柑桔绿霉病拮抗细菌的筛选, 鉴定及其抑制效果[J]. 中国生物防治学报, 2011, 27(2):284-288.
[4] 谷医林, 王远宏, 常若葵, 等. 解淀粉芽孢杆菌Lj1诱导黄瓜抗白粉病的研究[J]. 农药学学报, 2013, 15(3):293-298.
[5] 张立强, 黄慧琴, 崔莹, 等. 一株抗根结线虫芽孢杆菌的分离筛选及鉴定[J]. 热带作物学报, 2014, 35(9):1825-1829.
[6] Cawoy H, Bettiol W, Fickers P, et al. Bacillus-based biological control of plant diseases//Stoytcheva M. Pesticides in the Modern World-Pesticides Use and Management[M]. Croatia:InTech, 2011, 273-302.
[7] 刘永峰, 陈志谊, 周明国, 等. 枯草芽孢杆菌Bs-916的抑菌活性及其抑菌物质初探[J]. 农药学学报, 2007, 9(1):92-95.
[8] 东秀珠, 蔡妙英. 常见细菌系统鉴定手册[M]. 北京:科学出版社, 2001.
[9] 朱飞舟, 陈利玉, 陈汉春. 16S rRNA基因序列分析法测定病原细菌[J]. 中南大学学报(医学版), 2013, 38(10):1035-1041.
[10] Yamamoto S, Harayama S. PCR amplification and direct sequencing of gyrB genes with universal primers and their application to the detection and taxonomic analysis of Pseudomonas putida strains[J]. Applied and Environmental Microbiology, 1995, 61(3):1104-1109.
[11] 吴新平, 张文锋, 刘乃炽, 等. 农药田间药效试验准则(杀菌剂), 杀菌剂防治瓜类炭疽病[M]. 北京:中国农业出版社, 2008.
[12] Madhaiyan M, Poonguzhali S, Kwon S W, et al. Bacillus methylotrophicus sp. nov., a methanol-utilizing, plant-growth-promoting bacterium isolated from rice rhizosphere soil[J]. International Journal of Systematic and Evolutionary Microbiology, 2010, 60(10):2490-2495.
[13] 冯云利, 奚家勤, 马莉, 等. 烤烟品种NC297内生细菌中拮抗烟草黑胫病的生防菌筛选及种群组成分析[J]. 云南大学学报(自然科学版), 2011, 33(4):488-496.
[14] 黄霄, 陈波, 周登博, 等. 菌株BM-24的分离鉴定及对香蕉枯萎病菌的抑菌活性[J]. 植物保护学报, 2013, 40(2):121-127.
[15] Shan H Y, Zhao M M, Chen D X, et al. Biocontrol of rice blast by the phenaminomethylacetic acid producer of Bacillus methylotrophicus strain BC79[J]. Crop Protection, 2013, 44(2):29-37.
[16] 吕倩, 胡江春, 王楠, 等. 南海深海甲基型芽孢杆菌SHB114抗真菌脂肽活性产物的研究[J]. 中国生物防治学报, 2014, 30(1):113-120.
[17] 王洪梅, 吴云成, 沈标. 青枯病生防菌N5的特性及其生物学效应[J]. 土壤, 2013, 45(6):1082-1090.