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journal1 ›› 2017, Vol. 33 ›› Issue (2/3): 289-296.DOI: 10.16409/j.cnki.2095-039x.2017.02.021

• SCIENTIFIC NOTES • Previous Articles     Next Articles

Bioactivity Effects of Artemisia argyi Essential Oil on Survival and Protective Enzyme Activities of Tetranychus cinnabarinus(Acari: Tetranychidae)

MA Xinyao1, LIU Yaohua1, CHENG Zuohui2, LI Rui1, LI Shengcai1   

  1. 1. College of Agriculture, Shanxi Agricultural University, Taigu 030801, China;
    2. College of Arts and Sciences, Shanxi Agricultural University, Taigu 030801, China
  • Received:2016-10-22 Online:2017-06-08 Published:2017-04-08

Abstract: The bioactivity of Artemisia argyi essential oil on Tetranychus cinnabarinus was investigated using slide-dip and fumigation methods. The activities of three protective enzymes (SOD, POD, and CAT) and protease in female adults exposed to essential oil at sublethal dosages (LD10, LD20, and LD30) and median lethal dosage (LD50) were analyzed at 4, 8, 12, 16, 20 and 24 hours post-treatment. The results showed that female adults died at 21.48%, 27.41%, and 77.78% at 24, 48, and 72 h post-treatment at 2.0% concentration, and the LD50 values were 23.03%, 13.25%, and 0.35%, respectively. Moreover, the fumigation effect of A. argyi essential oil was stronger on eggs than on female adults, and the ID50andLD50 values were 0.21 and 8.93 μL/L, respectively. SOD and POD were activated, and CAT and protease activities were inhibited after treatment with different doses (LD10, LD20, and LD30). Treatment at LD10, LD20, and LD30 for 8 hours increased SOD activities by 0.43, 1.29, and 1.60 times in comparison with the control group, respectively; POD was activated by 54.64%, 31.96% and 5.15%, respectively; CAT was inhibited by 27.03%, 20.46% and 31.65%, respectively; Protease inhibition rates were 21.53%, 29.16% and 45.09%, respectively. The results indicate that treatment ofA. argyi essential oil at sublethal dosages can disturb the dynamic balance of protective enzymes and inhibit protease activity, which disturbs normal metabolism and produces toxic effects on T. cinnabarinus adult females. These results support the use of A. argyi essential oil as a control agent against T. cinnabarinus.

Key words: Artemisia argyi essential oil, Tetranychus cinnabarinus, bioactivity, sublethal dosage, protective enzymes

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