Identification of an Isolate of Ostrinia furnacalis Nucleopolyhedrovirus and Its Bioactivity against Spodoptera frugiperda Larvae

doi:10.16409/j.cnki.2095-039x.2019.05.031

Abstract

Abstract: In this study, we conducted molecular identification, morphological characterisation and bioactivity of a new nucleopolyhedrovirus with wide host range isolated from Ostrinia furnacalis (Guenée) in Enshi, Hubei Province. The results indicated that the newly isolated nucleopolyhedrovirus was homologous to Rachiplusia ou nucleopolyhedrovirus and Anagrapha falcifera nucleopolyhedrovirus, while Kimura 2-parameter distances did not support it was the same species as these two viruses. The median lethal concentration of Ostrinia furnacalis nucleopolyhedrovirus was 3.86 times of that of Mamestra brassicae nucleopolyhedrovirus. These results will lead to the development of efficacious virus-based bioinsecticides against Spodoptera frugiperda.

Key words: nucleopolyhedrovirus, Spodoptera frugiperda, bioactivity

CLC Number:

S476.1

LEI Chengfeng, JIANG Ganming, PENG Ling, YANG Jian, HU Jia, SUN Xiulian. Identification of an Isolate of Ostrinia furnacalis Nucleopolyhedrovirus and Its Bioactivity against Spodoptera frugiperda Larvae[J]. Chinese Journal Of Biological Control, 2019, 35(5): 741-746.

References

[1] Todd E L, Poole R W. Keys and illustrations for the armyworm moths of the noctuid genus Spodoptera Guenée from the Western Hemisphere[J]. Annals of the Entomological Society of America, 1980, 73(6):722-738.
[2] CABI. Spodoptera frugiperda (fall armyworm)[R]. Wallingford, UK:CAB International, 2016.
[3] Prasanna B M, Huesing J E, Eddy R, et al. Fall Armyworm in Africa:A Guide for Integrated Pest Management, 1st edition[M]. México:USAID, CIMMYT, 2018.
[4] Nakweya G. Global actions needed to combat fall armyworm[EB/OL]. (2018-09-28). https://www.scidev.net/sub-saharan-africa/farming/news/global-actions-combat-fall-armyworm.html.
[5] FAO. FAW monitoring & early warning system (FAMEWS)[R]. Rome:FAO, 2018.
[6] 杨普云, 朱晓明, 郭井菲, 等. 我国草地贪夜蛾的防控对策与建议[J]. 植物保护, 2019, 45(4):1-6.
[7] Greene G L, Leppla N C, Dickerson W A. Velvet bean caterpillar (Lepidoptera:Noctuidae) rearing procedure and artificial medium[J]. Journal of Economic Entomology, 1976, 69:487-488.
[8] Yang S L, Zhao L J, Ma R P, et al. Improving baculovirus infectivity by efficiently embedding enhancing factors into occlusion bodies[J]. Applied and Environmental Microbiology, 2017, 83(14):e00595.
[9] Jehle J A, Lange M, Wang H L, et al. Molecular identification and phylogenetic analysis of baculoviruses from Lepidoptera[J]. Virology, 2006, 346:180-193.
[10] Nei M, Kumar S. Molecular evolution and phylogenetics[M]. New York:Oxford University Press, 2000.
[11] Kumar S, Tamura K, Nei M. MEGA3:integrated software for molecular evolutionary genetics analysis and sequence alignment[J]. Brief Bioinform, 2004, 5, 150-163.
[12] LeOra Software, Software Polo-Plus, POLO for Windows[CP]. Petaluma, CA, 2007.
[13] Robertson J L, Jones M M, Olguin E et al. Bioassays with Arthropods (3rd Edition)[M]. Boca Raton, FL:CRC Press, 2017.
[14] Wennmann J T, Keilwagen J, Jehle J A. Baculovirus Kimura two-parameter species demarcation criterion is confirmed by the distances of 38 core gene nucleotide sequences[J]. Journal of General Virology, 2018, 99(9):1307-1320.
[15] 殷永升, 常金玉, 裴美云, 等. 亚洲玉米螟颗粒体病毒利用研究[J]. 山西农业科学, 1993, 21(l):52-57.
[16] Federici B A. Naturally occurring baculoviruses for insect pest control//Hall F R, Menn J J, eds. Biopesticides:Use and Delivery[M]. Totowa, NJ:Humana Press, 1999.
[17] Puttler B, Hostetter D L. A New broad host spectrum Nuclear Polyhedrosis Virus isolated from a celerylooper, Anagrapha falcifera (Kirby), (Lepidoptera:Noctuidae)[J]. Environmental Entomology, 1991, 20(5):1480-1488.
[18] Doyle C J, Hirst M L, Cory J S. et al. Risk assessment studies:detailed host range testing of wild-type cabbage moth, Mamestra brassicae (Lepidoptera:Noctuidae), nuclear polyhedrosis virus[J]. Applied and Environmental Microbiology, 1990, 56(9):2704-2710.