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journal1 ›› 2015, Vol. 31 ›› Issue (6): 876-881.DOI: 10.16409/j.cnki.2095-039x.2015.06.009

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Effect of Metarhizium anisopliae on Protective Enzyme and Detoxification Enzyme in the Midgut of Locusta migratoria manilensis

ZHANG Yanfeng1,2,3, WANG Zhenghao2,4, NONG Xiangqun2,3, CAO Guangchun2,3, ZHAO Li1, WANG Guangjun2,3, ZHANG Zehua2,3   

  1. 1. College of Agronomy, Xinjiang Agricultural University, Urumqi 830052, China;
    2. State Key Laboratory of Plant Diseases and Insect Pests/Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100081, China;
    3. Scientific Observation and Experimental Station of Pests in Xilingol Rangeland, Ministry of Agriculture, Xilinhot 026000, China;
    4. College of Pratacultural Science, Gansu Agricultural University, Lanzhou 730070, China
  • Received:2015-04-08 Online:2015-12-08 Published:2015-12-17

Abstract: To study the effects of Metarhizium anisopliae infection on the activity of protective enzymes (SOD, POD, CAT, PO) and detoxification enzymes (AchE, ESTs) in the midgut of Locusta migratoria manilensis for understanding the mode of action of M. anisopliae, the activity of those enzymes in the midgut of L. migratoria manilensis was monitored at 1, 2, 3, 4, and 5 d after infected by M. anisopliae using spectrophotometry. The results indicated that the activities of SOD, POD, CAT increased firstly, then decreased, and increased again at the last phase, but that of PO showed an increasing-decreasing-increasing-decreasing tendency. AchE showed the same trend like SOD, its activity also increased firstly, then decreased, and increased finally. ESTs changed differently with others overall, its activity decreased gradually and went down to the level of control at the fifth day. These results suggested that the activities of SOD, POD, CAT, PO, AchE and ESTs changed differently after L. migratoria manilensis was infected by M. anisopliae, it will contribute to uncover the mode of action of M. anisopliae in the midgut of hosts and the immune mechanism of hosts against pathogens.

Key words: Metarhizium anisopliae, Locusta migratoria manilensis, midgut, protective enzymes, detoxifying enzymes

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