Identification of Antagonistic Fungus against Sphaerotheca fuliginea and Evaluation of Its Biocontrol Efficacy

doi:10.16409/j.cnki.2095-039x.2017.03.019

Abstract

Abstract: The antagonistic fungus against the pathogen Sphaerotheca fuliginea were isolated and assessed in this paper. The resulted showed that 21 strains of antagonistic fungus were isolated from the edge of cucumber tissue infected by S. fuliginea. Among all the isolated strains, the strain JK-13 presented the highest control efficacy in vitro and greenhouse experiments. The inhibitory rate on S. fuliginea spores germination was 83.93% after treated by strain JK-13 fermentation broth for 48 h. The control efficacy of strain JK-13 fermentation broth on S. fuliginea were 88.02% and 76.34% after treated for 7 d by leaf disc and pot experiments, respectively. Based on the morphological characteristic and molecular identification, the strain JK-13 was identified as Fusarium solani.

Key words: cucumber powdery mildew, antagonistic fungus, identification, biological control

CLC Number:

S476

LU Xingrong, CHEN Dawei, ZHANG Shuwu, LIU Jia, XU Bingliang. Identification of Antagonistic Fungus against Sphaerotheca fuliginea and Evaluation of Its Biocontrol Efficacy[J]. journal1, 2017, 33(2/3): 427-432.

References

[1] Dik A J, VerhaarM A, Berlanger R R. Comparison of three biological control agents against cucumber powdery mildew (Sphaerotheca fuliginea) in semi-commercial-scale glasshouse trials[J]. European Journal of Plant Pathology, 1998, 104(4): 413-423.
[2] Khan M R, Khan M W. Effects of intermittent ozone exposures on powdery mildew of cucumber[J]. Environmental and Experimental Botany, 1999, 42(3): 163-171.
[3] 杨春林, 席亚东, 刘波微, 等. 哈茨木霉一菌株对黄瓜白粉病的防治效果及其促生作用[J]. 中国生物防治, 2008, 24(增刊): 55-58.
[4] 孙睿揆, 王志远, 飞兴文, 等. 辣椒白粉病生防菌株 Bs2 的筛选鉴定及田间防效研究[J]. 西南农业学报, 2011, 24(3): 974-978.
[5] 伊艳杰, 张长付, 时玉, 等. 小麦白粉菌拮抗真菌的筛选、鉴定及生防效果[J]. 中国农学通报, 2010, 26(14): 273-276.
[6] 方中达. 植病研究法[M]. 北京: 中国农业出版社, 2007.
[7] 陈波, 张燕. 九种植物提取物对小麦白粉病菌孢子萌发的影响[J]. 湖北农业科学, 2015, 54(9): 2127-2131.
[8] Cohen R. A leaf disk assay for detection of resistance of melons to Sphaerotheca fuliginea race 1[J]. Plant Disease, 1993, 77(5): 513-517.
[9] 刘学, 朱春雨, 徐文平, 等. 农药室内生物测定试验准则杀菌剂第11部分: 防治瓜类白粉病试验盆栽法: NY/T 1156.11-2008[S]. 北京: 中国农业出版社, 2008.
[10] 魏景超. 真菌鉴定手册[M]. 上海: 上海科学技术出版社, 1979.
[11] Nakada M, Tanaka C, Tsunewaki K, et al. RFLP analysis for species separation in the genera Bipolaris and Curvularis[J]. Mycoscience, 1994, 35(3): 271 -278.
[12] 申光辉, 薛泉宏, 张晶, 等. 草莓根腐病拮抗真菌筛选鉴定及其防病促生作用[J]. 中国农业科学, 2012, 45(22): 4612-4626.
[13] 范瑛阁, 龚明福, 朱丽霞. 黄瓜白粉病菌生防菌筛选及生防机制初步研究[J]. 北方园艺, 2010(14): 150-153.
[14] Gafni A, Calderon C E, Harris R, et al. Biological control of the cucurbit powdery mildew pathogen Podosphaera xanthii by means of the epiphytic fungus Pseudozyma aphidis and parasitism as a mode of action[J]. Frontiers in Plant Science, 2015, 11(6): 132.
[15] 屈佳玉. 大豆胞囊线虫生防菌的初步研究[D]. 哈尔滨: 黑龙江大学, 2008.
[16] 付洁, 侯军, 郝双红. 禾谷镰刀菌毒素的抑菌除草活性测定[J]. 陕西农业科学, 2006(1): 3-4.
[17] 段玉玺, 曲泽岚, 王媛媛, 等. 南方根结线虫生防镰刀菌菌株筛选[J]. 农药, 2010, 49(8): 607-609.
[18] Boari A, Vurro M. Evaluation of Fusarium spp. and other fungi as biological control agents of broomrape (Orobanche ramose)[J]. Biological Control, 2004, 30(2): 212-219.
[19] 李健, 李岩, 高兴祥, 等. 马唐生防菌厚垣孢镰刀菌ZC201301的生物学特性研究[J]. 草业学报, 2016, 25(3): 234-239.