Application of Microbial Biocontrol Agents Combined with Organic Material Addition for Combating Strawberry Gray Mold

doi:10.16409/j.cnki.2095-039x.2020.05.018

Abstract

Abstract: To solve the unstable effect of microbial biological control agents (BCAs) on strawberry gray mold, field experiments were performed to determine the control effects of different microbial BCAs combined with organic material on strawberry gray mold and the number of soil microorganisms. There were 15 treatments in this experiment. Microbial BCAs included Bacillus subtilis, Trichoderma, effective microorganisms (EM), half-dose Bacillus subtilis+Trichoderma (1/2BT) and no BCA control (CK). organic materials (rice bran and soybean meal powder, RB) included 3 levels:no add, 1/2RB (15 g/m² rice bran +60 g/m² soybean meal powder) and RB (30 g/m² rice bran +120 g/m² soybean meal powder). After 55 days of transplanting, the components of each treatment were evenly broadcasted around the root, and subsequently covered plastic film, moisture was kept with drip irrigation. The results showed that Trichoderma and EM could effectively reduce the incidence of gray mold. The order of the control effects of BCAs was EM > Tr > Bs > 1/2BT with the same amount of organic material addition. The control effects of single Trichoderma and EM were 60.00%-68.00% in the mild disease test site 1 and 16.39%-29.01% in the serious disease test site 2. The treatment of EM+RB showed the highest prevention effect, which was up to 90.00%. The control effect of BCAs were significantly increased by adding the full amount of rice bran and soybean meal powder. The order of the control effects of BCAs with organic material addition was BCA+RB > BCA+1/2RB > BCA. The application of Tr+RB and EM+RB increased the control effects by 0.17-1.1 times and 0.32-1.84 times comparing to single BCAs, respectively. Meanwhile, the soil microbial population was positively regulated by application of organic materials. Application of 1/2RB and RB increased the microbial population by 0.43~8.86 times and 0.93~32.72 times, respectively. These results suggested that the application of Trichoderma or EM could effectively control strawberry gray mold in actual production, and the addition of sufficient organic materials could increase the soil microbial abundance, which might consequently improve the control effect.

Key words: strawberry, microbial biological control agents, gray mold, rice bran, soybean meal powder

CLC Number:

S436.639

HUANG Jiexue, WANG Xiaolin, WU Jie, JI Muxiang. Application of Microbial Biocontrol Agents Combined with Organic Material Addition for Combating Strawberry Gray Mold[J]. Chinese Journal Of Biological Control, 2020, 36(5): 802-810.

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