Identification of an Aschersonia Isolate and Effects of Nutrition and Temperature on Its Mycelial Growth and Sporulation

doi:10.16409/j.cnki.2095-039x.2018.01.011

Abstract

Abstract: Aschersonia species are important entomopathogenic fungi with potential as biopesticides against whiteflies and coccids. An Aschersonia strain, named ZKB-1, was isolated from parasitized coccids collected from a citrus orchard in Gannan in Jiangxi Province. Based on the morphological characteristics and molecular analysis of ITS-rDNA gene, the isolate ZKB-1 was identified as Aschersonia placenta. The optimum carbon and nitrogen source and temperature for mycelial growth and sporulation in this fungus were determined. It was shown that starch and yeast extract powder were the optimum carbon and nitrogen source with mycelial growth speed of 0.56 and 0.60 mm/d, respectively, while glucose and ammonium sulfate were the least favorable ones with mycelial growth speed of 0.36 and 0.44 mm/d, respectively. Conidial yields grown on glucose and potassium nitrate were the highest, reaching 7.78×10⁷ and 1.40×10⁸ conidia/cm² on the 21th day, respectively, while the yields grown on starch and peptone were the lowest, being 0.63×10⁷ and 1.20×10⁷ conidia/cm² on the 21th day, respectively. The optimum temperature was 28 and 25℃for mycelial growth and for sporulation, respectively. Our results can provide a reference for the industrial fermentation of A. placenta.

Key words: biological control, carbon/nitrogen sources, temperature, mycelial growth, sporulation

CLC Number:

S476

LI Qian, XIE Ming, LI Qiang, ZHANG Yanjun, MAO Xinrong, QIU Weiliang. Identification of an Aschersonia Isolate and Effects of Nutrition and Temperature on Its Mycelial Growth and Sporulation[J]. journal1, 2018, 34(1): 92-98.

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