Cloning and Induced Expression of Sugar Transporter Gene in Parasitoid, Encarsia formosa Gahan

doi:10.16409/j.cnki.2095-039x.2018.02.011

Abstract

Abstract: Encarsia formosa Gahan is a kind of endoparasitoid world widely used for control of pest whiteflies. Sugar supplement prolong longevity and/or increase their egg production; however, the sugar transporter gene and the induced expression in E. formosa was unclear. In this present study, the sugar transporter gene named EfST1 was cloned; sequence alignment and phylogenetic analysis revealed that EfST1 shared more than 65% homology with other parasitoids. Real-time PCR showed that EfST1 was significantly expressed by feeding different concentrations of glucose. After feeding sugar for different periods (2-48 h) on 10% glucose, EfST1 gene expression was found to be increased compared to the water control. Besides, there were no significant differences between the 2 h and 48 h feeding treatments. In conclusion, the EfST1 gene was probably related to sugar supplement and transportation; and the gene expression induced by different sugar concentrations was not greatly fluctuated. The results will provide a basis for studying the nutrition supplement and transportation mechanism during the biological control of parasitoids.

Key words: Encarsia formosa, Bemisa tabaci, CODEHOP, sugar transporter gene, expression analysis

CLC Number:

S476.3

LIU Yicong, ZHANG Youjun, WU Qingjun, XIE Wen, WANG Shaoli. Cloning and Induced Expression of Sugar Transporter Gene in Parasitoid, Encarsia formosa Gahan[J]. journal1, 2018, 34(2): 247-253.

References

[1] Burger J, Hemerik L, Lenteren J C, et al. Reproduction now or later:optimal host-handling strategies in the whitefly parasitoid Encarsia formosa[J]. Oikos, 2004, 106(1):117-130.
[2] 王伟, 刘万学, 程立生, 等. 取食不同糖分对卵育型寄生蜂潜蝇姬小蜂雌蜂寿命和卵子发生的影响[J]. 昆虫学报, 2012, 55(8):964-970.
[3] Williams Ⅲ L, Deschodt P, Pointurier O, et al. Sugar concentration and timing of feeding characteristics and survival of a parasitic wasp[J]. Journal of Insect Physiology, 2015, 79(1):10-18.
[4] Hirose Y, Mitsunaga T, Yano E, et al. Effects of sugars on the longevity of adult females of Eretmocerus eremicus and Encarsia formosa (Hymenoptera:Aphelinidae), parasitoids of Bemisia tabaci and Trialeurodes vaporariorum (Hemiptera:Alyerodidae), as related to their honeydew feeding and host feeding[J]. Applied Entomology and Zoology, 2009, 44(1):175-181.
[5] Zhang Y B, Yang N W, Wang J J, et al. Effect of six carbohydrate sources on the longevity of a whitefly parasitoid Eretmocerus hayati (Hymenoptera:Aphelinidae)[J]. Journal of Asia-Pacific Entomology, 2014, 17(4):723-728.
[6] Yang Z Z, Xia J X, Pan H P, et al. Genome-wide characterization and expression profiling of sugar transporter family in the whitefly, Bemisia tabaci (Gennadius) (Hemiptera:Aleyrodidae)[J]. Frontiers in Physiology, 2017, 8:322.
[7] Ge L Q, Jiang Y P, Xia T, et al. Silencing a sugar transporter gene reduces growth and fecundity in the brown planthopper, Nilaparvata lugens (Stål) (Hemiptera:Delphacidae)[J]. Scientific Reports, 2015, 5:12194.
[8] Govindaraj L, Gupta T, Esvaran V G, et al. Genome-wide identification, characterization of sugar transporter genes in the silkworm Bombyx mori and role in Bombyx mori nucleopolyhedrovirus (BmNPV) infection[J]. Gene, 2016, 579(2):162-171.
[9] Price D R, Gatehouse J A. Genome-wide annotation and functional identification of aphid GLUT-like sugar transporters[J]. BMC Genomics, 2014, 15:647.
[10] Kikuta S, Nakamura Y, Hattori M, et al. Herbivory-induced glucose transporter gene expression in the brown planthopper, Nilaparvata lugens[J]. Insect Biochemistry and Molecular Biology, 2015, 64(1):60-67.
[11] Lourenço P M L, Almeida T, Mendonça D, et al. Searching for nitrile hydratase using the consensus-degenerate hybrid oligonucleotide primers strategy[J]. Journal of Basic Microbiology, 2004, 44(3):203-214.
[12] Henikoff S, Henikoff J G, Alford W J, et al. Automated construction and graphical presentation of protein blocks from unaligned sequences[J]. Gene,1995, 163(2):17-26.
[13] Rose T M, Schultz E R, Henikoff J G, et al. Consensus-degenerate hybrid oligonucleotide primers for amplification of distantly related sequences[J]. Nucleic Acids Research, 1998, 26(7):1628-1635.
[14] Chakravorty S, Vigoreaux J O. Amplification of orthologous genes using degenerate primers[J]. Methods in Molecular Biology, 2010, 634:175-185.
[15] 赵运胜,卜友泉, 廖飞. 用简并引物快速克隆苛求芽孢杆菌尿酸酶的编码序列[J]. 重庆医科大学学报, 2017, 42(2):219-224.
[16] Cheng Q, Cao Y, Jiang C, et al. Identifying secreted proteins of Marssonina brunnea by degenerate PCR[J]. Proteomics, 2010, 10(13):2406-2417.
[17] Chouhy D, Kocjan B J, Staheli J P, et al. Detection of novel Betapapillomaviruses and Gammapapillomaviruses in eyebrow hair follicles using a single-tube ‘hanging droplet’ PCR assay with modified pan-PV CODEHOP primers[J]. Journal of General Virology, 2018, 99(1):109-118.
[18] 孔卫青, 杨金宏. 家蚕糖转运蛋白BmST5基因的克隆与转录活性检测[J]. 河南农业科学, 2010(8):126-129.
[19] 孔卫青, 杨金宏. 家蚕糖转运蛋白基因BmST3的克隆及序列分析与表达研究[J]. 蚕业科学, 2010, 36(4):604-609.
[20] Kikuta S, Kikawada T, Hagiwara-Komoda Y, et al. Sugar transporter genes of the brown planthopper, Nilaparvata lugens:A facilitated glucose/fructose transporter[J]. Insect Biochemistry and Molecular Biology, 2010, 40(11):805-813.
[21] Zinke I, Schutz C S, Katzenberger J D, et al. Nutrient control of gene expression in Drosophila:microarray analysis of starvation and sugar-dependent response[J]. EMBO Journal, 2002, 21(22):6162-6166.