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journal1 ›› 2018, Vol. 34 ›› Issue (2): 254-258.DOI: 10.16409/j.cnki.2095-039x.2018.02.012

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The Establishment of the CRISPR/Cas9 System in Bemisia tabaci and the Application in the Identification of Temperature Tolerance Function of Transient Receptor Potential Gene

WANG Yuan1, LÜ Zhichuang2, WAN Fanghao1,2,3   

  1. 1. College of Agriculture and Plant Protection, Qingdao Agricultural University, Qingdao 266109, China;
    2. State Key Laboratory for Biology of Plant Diseases and Insect Pests/Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
    3. Center for Management of Invasive Alien Species Ministry of Agricultulture, Beijing 100193, China
  • Received:2017-06-07 Online:2018-04-08 Published:2018-04-13

Abstract: Whiteflies could quickly spread around the world and become the important pests, and one of the main reasons is its strong resistance to the temperature change. As the change of environment, a lot of cell surface receptors and ion channels in the body could be stimulated, then affected the animal's behavior. Among them, the transient receptor potential (TRP) is a key regulation factor in the insect perception of the environment temperature. This research mainly explored a simple and highly effective method, generating and detecting mutations of TRP gene in Bemisia. tabaci through CRISPR/cas9 system, to identify the function of TRP gene in temperature feeling of B. tabaci Here, we expounded the experimental process of injecting sgRNA in red eye phase pupa of B. tabaci MED, being induced highly efficient mutagenesis of desired target genes, and detected statistical time of heat knockdown or chill coma recovery. The result showed that the sensitivity of the temperature change of injected B. tabaci MED declined significantly, and couldn't adjust temperature adaptability, leading to the decrease of temperature tolerance under hot or cold stress environments.

Key words: Bemicia tabaci MED, gene mutations, thermoreceptor, TRP gene

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