The Phenotype Determination of Bacillus thuringiensis JQ23 and Its Control Efficiency against Bradysia odoriphaga

doi:10.16409/j.cnki.2095-039x.2016.03.007

Abstract

Abstract: Bradysia odoriphaga is an important pest of Chinese chive production. A new Bacillus thuringiensis strain JQ23 was isolated from the soil collected from Henan province by antibiotic method. GEN III Biolog bacterial identification system showed that JQ23 strain can use 13 kinds of carbon sources, and is sensitive to 9 chemical substances. Bioassay showed that JQ23 strain was of high insecticidal activity against B. odoriphaga, with 72 h LC₅₀ of 8.3804×10⁶ spore/mL against 2nd instars of B. odoriphaga. The pot tests showed that, continuous dripping application of JQ23 at 1×10⁷ spore / mL for three times at intervals of 7 d, the final control reached 86.28%. In field plot experiment in spring, continuous dripping application of JQ23 at 1×10⁸ spore/mL for three times at intervals of 7―10 d, the final control was 66.75%. These results show that the JQ23 strain can achieve an effective control of B. odoriphaga.

Key words: Bradysia odoriphaga, Bacillus thuringiensis, control effect

CLC Number:

SONG Jian, CAO Weiping, Zhang Haijian, Wang Jinyao, Feng Shuliang, DU Lixin. The Phenotype Determination of Bacillus thuringiensis JQ23 and Its Control Efficiency against Bradysia odoriphaga[J]. journal1, 2016, 32(3): 326-331.

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