Analysis of Symbiotic Bacteria of Propylea japonica Larvae

doi:10.16409/j.cnki.2095-039x.2018.02.021

Abstract

Abstract: Symbiotic relationships between bacteria and insects are common in nature. Symbiotic bacteria participate in host's physiological processes of reproduction, metabolism and detoxification. Propylaea Japonica (Coleoptera:Coccinellidae) is an important natural enemy of insect pests in agroecosystems. To clarify the community structure and diversity of bacteria in this insect, the V3-V4 regions of 16S rDNA genes of bacteria were investigated in the beetle larvae using high-throughput sequencing of Illumina MiSeq techniques and bioinformatics methods. A total of 3910 OTUs were obtained, of which sequences of a length 360-480 bp accounts for 99.5%. The bacteria were identified to 37 phylum, 75 classes, 143 orders, 261 families, and 605 genera. Bacteria species of Proteobacteria (51.84%), Firmicutes (21.81%), Actinobacteria (11.35%), Cyanobacteria (7.06%), and Bacteroidetes (4.49%) were dominant in P. japonica. On the genus level, Staphylococcus (16%), Acinetobacter (8.54%), Paracocccus (4.93%), Pseudomonas (3.59%), Boechera gunnisoniana (3.34%), Corynebacterium (2.18%), Cronobacter (2.04%), Kocuria (1.50%), Streptococcus (1.15%), and Bacillus (1.11%) were dominant. The preliminarily defined structure and composition of symbiotic bacteria in the P. japonica larvae provide a basis for improving its artificial rearing and a new perspective for the safety evaluation of transgenic plant for P. japonica.

Key words: Propylea japonica, symbiotic bacteria, Illumina MiSeq techniques, advantage flora

CLC Number:

S476.2

WU Linke, ZHANG Shuai, LUO Junyu, ZHANG Lijuan, LÜ Limin, ZHU Xiangzhen, WANG Li, CUI Jinjie. Analysis of Symbiotic Bacteria of Propylea japonica Larvae[J]. journal1, 2018, 34(2): 317-323.

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