Cloning a Peroxidase cDNA Sequence from the Oriental Armyworm, Mythimna separata Walker and Its Induction to Different Temperature Stress

doi:10.16409/j.cnki.2095-039x.2019.01.011

Abstract

Abstract: Peroxidase is a key antioxidant enzyme in insects and plays an important role in maintaining redox homeostasis in insects and protecting insects from oxidative damage. In this study, a novel peroxidase cDNA sequence MsPOD (GenBank accession:No. MH606240) was obtained from Mythimna separata by transcriptome sequencing technology. The full-length cDNA sequence MsPOD was 2433 bp, with ORF length of 2061 bp, encoding a polypeptide consisting of 686 amino acids. The peptide was characterized by a molecular mass of about 76.1 ku and an isoelectric point of about 5.68, and distinct domain of animal heme peroxidase cell adhesion protein. Amino acids alignment analysis showed that MsPOD amino acid sequence was close in homology to that of other Noctuidae insects, with the highest homology of 92% with that in Helicoverpa armigera. MsPOD expressed specifically at different developmental stages and in different tissues, the highest expression occurring at pupal stage and in salivary glands. Expression of MsPOD also varied with different temperatures, reaching the highest level at 12 h post treatment at 35℃. These results lay a foundation for the further study of protective effects of MsPOD and utilizing it to design molecular method to control M. separata.

Key words: Mythimna separata, peroxidase, cloning, temperature, induction

CLC Number:

S476

YANG Hang, WANG Xiaoyun, PEI Haiying, FAN Dong. Cloning a Peroxidase cDNA Sequence from the Oriental Armyworm, Mythimna separata Walker and Its Induction to Different Temperature Stress[J]. journal1, 2019, 35(1): 44-52.

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