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Chinese Journal Of Biological Control ›› 2020, Vol. 36 ›› Issue (1): 87-96.DOI: 10.16409/j.cnki.2095-039x.2019.06.014

• RESEARCH REPORTS • Previous Articles     Next Articles

Cloning and Sequence Analysis of Two P450 Genes and Expression of the Genes in Sogatella furcifera under Insecticides Stress

JIA Zeyan1, ZHOU Cao2, ZENG Qinghui1, SHI Min1, YANG Hong2,3, JIN Daochao2   

  1. 1. Agricultural College of Guizhou University, Guiyang 550025, China;
    2. Institute of Entomology, Guizhou University/Provincial Key Laboratory for Agricultural Pest Management of Mountainous Regions, Guiyang 550025, China;
    3. College of Tobacco Science, Guizhou University, Guiyang 550025, China
  • Received:2019-04-03 Online:2020-02-08 Published:2020-02-26

Abstract: Cytochrome P450 is a supergene family, which is closely related to insecticides resistance. Based on the transcriptome data of Sogatella furcifera, two P450 genes, SfCYP4DE1 and SfCYP353D1v2, were cloned. SfCYP4DE1 and SfCYP353D1v2 encode 534 and 471 amino acids with molecular weight of 61.567 and 53.762 kDa and theoretical isoelectric point of 9.18 and 8.74, respectively. Domain analysis showed that the amino acid sequences of SfCYP4DE1 and SfCYP353D1v2 contained the common sequences of 5 P450 genes. The relative expression levels of SfCYP4DE1 and SfCYP353D1v2 were determined by RT-qPCR at 48h post treatment of 3rd instar S. furcifera with different concentrations (LC10, LC25, LC50, and LC90) of thiamethoxam, buprofezin and avermectin. The relative expression of SfCYP4DE1 gene was significantly lower in the insects treated with thiamethoxam and buprofezin at both LC10 and LC25 than that in the control insects. However, with the further increase of insecticide concentration, the expression of SfCYP4DE1 gene showed an increasing trend. In contrast, when the insects were treated with avermectin at LC10 and LC25, the expression of SfCYP4DE1 gene was significantly higher than that in the control insects; and the expression level increased with the increase of avermectin concentration. For the SfCYP353D1v2 gene, expression was induced by different concentrations of thiamethoxam in comparison with the control, and was significantly higher in the LC10, LC50 and LC90 treatments than in the control. Treatment with buprofezin at LC10 significantly induced expression of the SfCYP353D1v2 gene, while treatment with avermectin at LC10 significantly inhibited the gene expression. However, treatment with avermectin at LC50 and LC90 significantly induced expression of the SfCYP353D1v2 gene. These results provide basic information for further study of the roles of P450 genes in detoxification and metabolism of insecticides in S. furcifera.

Key words: Sogatella furcifera, cytochrome P450, gene cloning, insecticide

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