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Chinese Journal Of Biological Control ›› 2020, Vol. 36 ›› Issue (2): 307-314.DOI: 10.16409/j.cnki.2095-039x.2020.02.018

• SCIENTIFIC NOTES • Previous Articles     Next Articles

Cloning and Sequences Analysis of Voltage-Gated Sodium Channel Gene in Sitobion avenae (Fabricius)

DUAN Wenbo1,2, WANG Hao1,2, LI Fen2,3, YIN Xinming1, WU Shaoying1,2,3   

  1. 1. College of Plant Protection, Henan Agricultural University, Henan Province, Zhengzhou 450002, China;
    2. College of Plant Protection, Hainan University, Haikou 570228, China;
    3. Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests, Hainan University, Ministry of Education, Haikou 570228, China
  • Received:2019-07-15 Online:2020-04-08 Published:2020-04-16

Abstract: The cDNA sequences of voltage-gated sodium channel gene of Sitobion avenae were cloned and its typical characteristics were identified, which laid a foundation for further studies of the molecular mechanism of pesticide resistance in S. avenae. The sequences were cloned by RT-PCR and analyzed by DNASTAR Lasergene 7.10 software. Two cDNA sequences were obtained, SaNav1 and SaNav2, with GenBank accession numbers of MN176137 and MN161584, respecitvely. The SaNav1 sequence was 3423 bp, encoding a total of 1141 amino acids. The SaNav2 sequence was 2874 bp, encoding a total of 958 amino acids. The homology alignment found that the similarity of SaNav1 and the part I sodium channel genes of Rhodolospi maidis (XP_026806613) and Myzus persicae (XP_022174241) were as high as 97.81% and 97.91%; the similarity of SaNav2 and the part II sodium channel genes of Rhodolospi maidis (ALB75309.1) and Myzus persicae (XP_022174237.1) were as high as 97.90% and 96.43%, respectively. The cloned sequences contain four homologous domains, each with six transmembrane segments (S1—S6) and a sodium channel-selective key residue "DENS". The sodium channel genes cloned in S. avenae will provide a basis for studying the role of sodium channel in resistance mechanism in S. avenae.

Key words: Sitobion avenae, sodium channel, gene cloning, knockdown resistance

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