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journal1 ›› 2018, Vol. 34 ›› Issue (5): 676-685.DOI: 10.16409/j.cnki.2095-039x.2018.05.005

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Molecular Cloning and Expression Analysis of Acyl-CoA Delta (11) Desaturase in the Diapausing Ladybird, Coccinella septempunctata L. (Coleoptera: Coccinellidae)

HAN Yanhua, LIU Mengyao, GAO Fei, WANG Manzi, ZHANG Hongzhi, KONG Lin, CHEN Hongyin, ZHANG Lisheng   

  1. State Key Laboratory for Biology of Plant Diseases and Insect Pests/Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China
  • Received:2018-04-17 Online:2018-10-08 Published:2018-10-15

Abstract: Acyl-CoA Δ11 desaturase is one of the fatty acid desaturases that are key enzymes in the synthesis pathway of unsaturated fatty acids. Based on the transcriptome database of Coccinella septempunctata L., we cloned the full length cDNA of a FadΔ11 gene using RT-PCR and RACE technique, which was named CsFadΔ11 (GenBank accession no. MF458996). CsFadΔ11 is 1447 bp in length and contains an open reading frame (ORF) of 1095 bp, encoding 364 amino acid residues. The putative protein is 42.99 kD with an isoelectric point (pI) of 8.87 and has no signal peptide. Amino acid sequence analysis showed that CsFadΔ11 contains three histidine boxes and six transmembrane domains, which are composite characteristics of a fatty acid desaturase. Meanwhile, CsFadΔ11 showed high homology with Zootermopsis nevadensis. The expression profile of this gene in different treatments was detected by quantitative real-time PCR. The results indicated that CsFadΔ11 was constently expressed in newly emerged adult, day 10 or 20 after diapause induction, early diapause, late diapause, post diapause and normal development adults, with the highest expression levels in early diapause and the second levels in post diapause. The change trend of the gene was consistent with the lipid content in different developmental stages of C. septempunctata. These results suggested that CsFadΔ11 gene may participate in lipid metabolism, and further affect the diapause development of C. septempunctata.

Key words: Coccinella septempunctata, acyl-CoA Delta (11) desaturase, gene cloning, gene expression, diapause, lipid accumulation

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