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journal1 ›› 2016, Vol. 32 ›› Issue (5): 657-665.DOI: 10.16409/j.cnki.2095-039x.2016.05.016

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Selection and Identification of Biological Control Bacillus Strains agaist Plant Bacterial Wilt Disease

ZHENG Xuefang, LIU Bo, ZHU Yujing, GE Cibin, LIU Guohong   

  1. Institute of Agricultural Bio-Resources Research, Fujian Academy of Agricultural Sciences, Fuzhou 350003, China
  • Received:2016-03-21 Online:2016-10-08 Published:2016-10-08

Abstract: Bacillus are the most promising candidates of biocontrol agents. In this study, 24 Bacillus-like strains isolated from different geographical regions were exhibited antagonistic activity against Ralstonia solanacearum by the inhibition zone method. Among them, six strains appeared to have high antagonistic activities with the inhibition zone diameters (IZD) more than 14.00 mm. Moreover, the stain FJAT-11709 had the highest antagonistic activity with an IZD of 14.78 mm. Subsequently, the six Bacillus-like strains were further evaluated their control efficiencies against tomato bacterial wilt disease through the pot experiments. The strains FJAT-20261 and FJAT-19700 displayed excellent control efficiencies, reaching up to 72.73% and 67.77%, respectively. Finally, the strains FJAT-20261 and FJAT-19700 were separately identified as Brevibacterium frigoritolerans and B. tequilensis on the basis of the morphological features, physiological characteristics, and 16S rRNA gene sequence analysis. It is demonstrated that B. frigoritolerans and B. tequilensis could inhibit growth of R. solanacearum. This work provided new biocontrol agents against bacterial wilt disease.

Key words: tomato bacterial wilt, Ralstonia solanacearum, Bacillus, biocontrol

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