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Chinese Journal of Biological Control ›› 2022, Vol. 38 ›› Issue (1): 196-204.DOI: 10.16409/j.cnki.2095-039x.2021.03.023

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Spatio-temporal Expression and Function of Cytochrome P450 Gene CYP4G113 in Conogethes punctiferalis

YUAN Xingxing, DONG Shaoqi, WANG Xinhui, GUO Xianru, WANG Gaoping, LI Weizheng, ZHANG Lijuan, ZHAO Man   

  1. College of Plant Protection, Henan Agricultural University, Zhengzhou 450002, China
  • Received:2021-12-21 Online:2022-02-08 Published:2022-03-01

Abstract: CYP family genes encode important detoxifying enzymes in insects. This study was designed to clarify the basic sequence characteristics, expression patterns and function of the CYP4G113 in the adaptive evolution of Conogethes punctiferalis. The open reading frame of C. punctiferalis CYP4G113 was cloned based on C. punctiferalis transcriptome data, and CYP4G113 expression levels were determined in various developmental stages and larval tissues using quantitative real-time PCR (qRT-PCR). CYP4G113 expression was silenced using RNA interference (RNAi) technology and the RNAi effect on C. punctiferalis growth and development was examined. The full-length C. punctiferalis CYP4G113 cDNA sequence, with an open reading frame of 1707 bp, was obtained (GenBank accession number:MT721971). This open reading frame encodes 568 amino acids, with a predicted molecular weight of approximate 64.59 kDa, and a theoretical isoelectric point of 8.54. qRT-PCR results showed that CYP4G113 was expressed in all developmental stages of C. punctiferalis, with the highest expression in 1st instar larvae, and the lowest expression in the eggs and 5th instar larvae. Meanwhile, the expression of CYP4G113 was the highest in the salivary gland among the tissues of 4th instar larvae. The survival and pupation rates of C. punctiferalis larvae on a suitable host plant, maize, were significantly reduced after CYP4G113 silencing. In C. punctiferalis, CYP4G113 is expressed at relatively high levels in young larval insects and is mainly expressed in the salivary gland. In addition, the growth and development of C. punctiferalis larvae are significantly affected by CYP4G113 silencing, indicating that this gene plays an important role in C. punctiferalis host adaptation. The results of this study will provide a new potential target gene for controlling insects using RNAi technology.

Key words: Conogethes punctiferalis, cytochrome P450, quantitative real-time PCR, RNAi, gene function

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