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Chinese Journal of Biological Control ›› 2021, Vol. 37 ›› Issue (3): 431-435.DOI: 10.16409/j.cnki.2095-039x.2021.03.037

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Isolation and Virulence of Entomogenic Nematodes against Tirathaba rufivena in Areca Plantation of Hainan

ZHONG Baozhu, Lü Chaojun, LI Chaoxu, QIN Weiquan   

  1. Coconut Research Institute, Chinese Academy of Tropical Agricultural Sciences/Hainan Engineering Research Center of Arecanut Industry, Wenchang 571339, China
  • Received:2020-09-20 Published:2021-06-16

Abstract: Tirathaba rufivena Walker is an important pest of areca palm, Areca catechu L.. In this study, Galleria mellonella bait technology was employed to investigate the distribution of entomopathogenic nematodes(EPN) isolated from soil samples collected in the main betel nut garden of Hainan province, and the virulence of target nematodes to T. rufivena was evaluated. EPNs were found in 12 of 150 collected soil samples with a 8% recovery rate. In Tunchang, Ding'an and Wanning, the recovery rates of nematodes were 12.90%, 11.76% and 10.00%, respectively. No EPN was detected in soil samples from Haikou and Baoting. The results showed that all the 12 EPN populations had certain virulence to the larvae of G. mellonella, but the virulence greatly varied (the corrected mortality ranged from 21.11 to 72.22% after treated 120 h). The virulence of Tc05, Wn01 and Wn09 which have higher pathogenicity to the 3rd instar larvae of T. rufivena was tested. The results revealed that after treated with 600 IJs/mL EPN suspensions, the corrected mortality rate of Wn01 was 86.67% after 24 h, and the LC50 was 283.70 IJs/mL. Tc05 and Wn09 had similar virulence to the larvae, with corrected mortality of 61.67% and 65.00%, and the LC50 was 504.75 and 502.70 IJs/mL, respectively. The above results indicated that Wn01, Tc05 and Wn09 EPN samples have potential on the control of T. rufivena larvae.

Key words: entomopathogenic nematode, areca plantation, Tirathaba rufivena, virulence

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