Screening of Highly Pathogenic Beauveria bassiana Strain and Its Virulence against Peanut Beet Armyworm

doi:10.16409/j.cnki.2095-039x.2018.02.013

Abstract

Abstract: To obtain high pathogenic Beauveria bassiana strains, four Beauveria isolates were identified and comparatively analyzed for their bio-characteristics and virulence. Control efficiency of high virulence strains was tested against peanut beet armyworm Spodoptera exigua (Hübner) in the field.The results showed significant differences in the biological characteristics and pathogenicity of the four B. bassiana strains, such as colony morphology, growth rate, and sporulation rate. The strain of the fastest growth and sporulation was F1, while the conidia of F6 strain were larger than those of other strains. F1 strain had the strongest pathogenicity to S. exigua, with LC₅₀ and LT₅₀ of 2.9383×10⁶ cfu/mL and 5.79 days, respectively; F8 strain seconded in pathogenicity. The virulences of strain F5 and F6 were relatively weak. In field test, F1 strain showed a control up to 75% against S. exigua, and strong colonization ability on peanut leaves.

Key words: Beauveria bassiana, Spodoptera exigua, peanut, virulence, colonization

CLC Number:

S476.1

MENG Xiangkun, ZHU Chao, YU Xin, LIU Yuanjun, HU Zhaoping, FAN Lingchao. Screening of Highly Pathogenic Beauveria bassiana Strain and Its Virulence against Peanut Beet Armyworm[J]. journal1, 2018, 34(2): 259-265.

References

[1] Shelton A M, Vandenberg J D, Wiley W T, et al. Efficacy and persistence of Beauvervia bassiana and other fungi for control of diamondback moth, Plutella xylostella, on cabbage seedlings[J]. Journal of Entomological Science, 1998, 33(2):142-151.
[2] Meyers J M, Stephen F M, Haavik L J, et al. Laboratory and field bioassays on the effects of Beauveria bassiana Vuillemin (Hypocreales:Cordycipitaceae) on red oak borer, Enaphalodes rufulus (Haldeman) (Coleoptera:Cerambycidae)[J]. Biological Control, 2013, 65(2):258-264.
[3] 蒲蛰龙, 李增智. 昆虫真菌学[M]. 安徽:科学技术出版社, 1996, 93-112.
[4] Ugine T A, Wraight S P, Sanderson J P. Microbial biological control potential of three strains of Beauveria bassianas against greenhouse shore fly Scatella tenuicosta:Assessment of virulence, mass production capacity, and effects on shore fly reproduction[J]. Biological Control, 2013, 65(3):348-356.
[5] 骆家玉. 白僵菌高孢粉生产技术及在农林业生物防治上的应用[J]. 安徽农学通报, 2015, 21(6):85-87.
[6] 李嘉, 任佳慧, 刘艳微, 等. 球孢白僵菌生物学特性研究进展及其杀虫效果展望[J]. 哈尔滨师范大学自然科学学报, 2014, 30(4):98-100
[7] 徐延平, 仇飞, 黄勃, 等. 一株小菜蛾高致病性球孢白僵菌菌株的筛选及其可湿性粉剂的田间药效试验[J]. 中国生物防治学报, 2013, 29(3):376-383.
[8] 张龙娃, 康克, 刘玉军, 等. 美国白蛾高毒力球孢白僵菌菌株筛选[J]. 昆虫学报, 2016, 59(1):111-118.
[9] 曹伟平, 宋健, 甄伟, 等. 球孢白僵菌生物学特性与其对不同昆虫侵染差异相关性分析[J]. 中国生物防治学报, 2013, 29(4):503-508.
[10] 张彬, 刘怀, 王进军, 等. 甜菜夜蛾研究进展[J]. 中国农学通报, 2008, 24(10):427-433.
[11] 苏宏华, 宋彬, 李丽, 等. 甜菜夜蛾的抗性及抗性机理研究进展应用[J]. 昆虫学报, 2012, 49(6):1659-1663.
[12] 胡安岭, 田兆丰, 高希武, 等. 球孢白僵菌与两种昆虫生长调节剂对甜菜夜蛾的联合作用[J]. 中国生物防治学报, 2015, 31(4):575-580.
[13] 王云滨, 董建臻, 宋入梅, 等. 甜菜夜蛾优良球孢白僵菌菌株的筛选[J]. 中国农学通报, 2007, 23(5):346-348.
[14] 米红霞, 刘吉平. 白僵菌生防菌株Bb10和4株家蚕来源白僵菌分离株的生物学特性研究[J]. 蚕业科学, 2010, 36(6):962-967.
[15] 刘吉平, 吕思行, 米红霞. 生物防治白僵菌与家蚕病原白僵菌的生物学特性初步比较[J]. 蚕业科学, 2011, 37(3):442-448.
[16] 苘娜娜, 马焕艳, 鲁华云, 等. 不同分子类型家蚕病原性白僵菌的分离及致病性试验[J]. 中国蚕业, 2015, 36(3):32-37.
[17] 黄剑, 吴文君. 利用EXCEL快速进行毒力测定中的致死中量计算和卡方检验[J]. 昆虫知识, 2004, 41(6):594-598.
[18] 宋健, 曹伟平, 张海剑, 等. 球孢白僵菌HFW-05菌株在蔬菜上的宿存研究[J]. 华北农学报, 2015, 30(s):336-341.
[19] 雷妍圆, 吕利华, 何余容, 等. 球孢白僵菌生物学特性与其对小菜蛾致病力相关性分析[J]. 中国生物防治, 2010, 26(2):143-148.
[20] 王登杰, 王海鸿, 张桃, 等. 三株球孢白僵菌侵染烟粉虱的比较生长动力学及其毒力[J]. 菌物学报, 2016, 35(5):577-585.
[21] 邹东霞, 史国英, 廖旺姣, 等. 桉蝙蛾高毒力白僵菌菌株筛选及鉴定[J]. 中国森林病虫, 2016, 35(5):1-4.
[22] 胡本进, 徐丽娜, 胡飞, 等. 稻蛀茎夜蛾高致病性球孢白僵菌菌株筛选[J]. 中国生物防治学报, 2015, 31(2):280-283.
[23] Wraight S P, Ramos M E. Delayed efficacy of Beauveria bassiana foliar spray applications against Colorado potato beetle:Impacts of number and timing of applications on larval and next-generation adult populations[J]. Biological Control, 2015, 83(2):51-67.
[24] 曹伟平, 宋健, 赵建江, 等. 球孢白僵菌与11种新型化学杀菌剂的相容性评价[J]. 中国生物防治学报, 2016, 32(6):749-755.
[25] 王胜华, 马长山, 孟宪科, 等. 1%甲维盐微乳剂与微生物农药混用防治甘蓝小菜蛾药效研究[J]. 农业开发与装备, 2015, 1:76-117.
[26] 宋龙腾, 于洪春, 王雨薇, 等. 卵孢白僵菌与农药混用对蛴螬防治效果研究[J]. 北方园艺, 2013, 33(1):131-134.
[27] 魏世誉, 王娟, 隋丽, 等. 球孢白僵菌在玉米中的定殖研究[J]. 哈尔滨师范大学自然科学, 2015, 31(6):122-126.
[28] Romero G R, Jurado I G, Delso C, et al. Transient endophytic colonizations of plants improve the outcome of foliar applications of mycoinsecticides against chewing insects[J]. Journal of Invertebrate Pathology, 2016, 136(3):23-31.
[29] Lingg A J, Donaldson M D. Biotic and abiotic factors affectingstability of Beauveria bassiana conidia in soil[J]. Journal of Invertebrate Pathology, 1981, 38(2):191-200.
[30] He Y R, Lu L H, Kuang Z B, et al. Effect of temperature and humidity on the virulence of beetle-derived Beauveria bassiana (Balsamo) Vuillemin (Deuteromycetes:Moniliales) against the daikon leaf Beetle, Phaedon brassicae Baly (Coleoptera:Chrysomelidae)[J]. Acta Entomologica Sinica, 2005, 48(5):679-686.
[31] Vassilakos T N, Athanassiou C G, Kavallieratos N G, et al. Influence of temperature on the insecticidal effect of Beauveria bassiana in combination with diatomaceous earth against Rhyzopertha dominica and Sitophilus oryzae on stored wheat[J]. Biological Control, 2006, 38(2):270-281.
[32] 解娇, 张正坤, 高星爱, 等. 球孢白僵菌不同剂型在玉米田间种群密度研究[J]. 吉林农业科学, 2013, 38(1):19-21.