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Chinese Journal Of Biological Control ›› 2019, Vol. 35 ›› Issue (6): 884-890.DOI: 10.16409/j.cnki.2095-039x.2019.06.009

• RESEARCH REPORTS • Previous Articles     Next Articles

Laboratory Assessment of Pathogenicity of Metarhizium anisopliae against Phenacoccus solani

HUANG Peng1, CHEN Hanxin2, YAO Jinai1, HUANG Jiancheng1, YU Deyi1   

  1. 1. Fujian Key Laboratory for Monitoring and Integrated Management of Crop Pests/Institute of Plant Protection, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China;
    2. Zhangzhou Institute of Agricultural Science, Zhangzhou 363005, China
  • Received:2019-06-12 Online:2019-12-08 Published:2019-12-18

Abstract: Phenacoccus solani is a newly recorded pest in China. The pest has been posing a serious damage in succulent plants in Zhangzhou, Fujian province. In this study, we assayed the virulence and control efficiency of Metarhizium anisopliae F061 and FM-03 strains against P. solani in the laboratory, so as to evaluate the biocontrol potential and application prospect of the two entomopathogenic fungi strains against the insect pest. Results showed that the corrected accumulative mortality of P. solani were 83.15% and 91.95% when the pest was exposed to 1×108 conidia/mL F061 and FM-03 in ten days, respectively. The Time-Dosage-Mortality (TDM) model showed a significant interaction of dosage-time effects of these two strains against P. solani, as indicated by the Pearson's chi-squared test and Hosmer-Lemeshow test. The dosage effect enhanced with the increase in infection time, or vice versa. The LC50 of F061 was 5.55×105 conidia/mL after an ten-day infection, and the LT50 of 1.0×108 conidia/mL was 5.04 d, both higher than those of FM-03 (1.11×105 conidia/mL and 4.67 d), indicating higher virulence of FM-03 than F061. In addition, the laboratory contro1 efficiency of these two strains also increased with the increase in infection time, being 74.06% and 81.32% for 1×108 conidia/mL of F061 and FM-03 in ten days, respectively. In conclusion, FM-03 can be preferentially used to control P. solani on succulent plants, while F061 can serve as an alternative strain.

Key words: Metarhizium anisopliae, Phenacoccus solani, laboratory virulence, laboratory contro1 efficiency

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