Laboratory Assessment of Pathogenicity of Metarhizium anisopliae against Phenacoccus solani

doi:10.16409/j.cnki.2095-039x.2019.06.009

Abstract

Abstract: Phenacoccus solani is a newly recorded pest in China. The pest has been posing a serious damage in succulent plants in Zhangzhou, Fujian province. In this study, we assayed the virulence and control efficiency of Metarhizium anisopliae F061 and FM-03 strains against P. solani in the laboratory, so as to evaluate the biocontrol potential and application prospect of the two entomopathogenic fungi strains against the insect pest. Results showed that the corrected accumulative mortality of P. solani were 83.15% and 91.95% when the pest was exposed to 1×10⁸ conidia/mL F061 and FM-03 in ten days, respectively. The Time-Dosage-Mortality (TDM) model showed a significant interaction of dosage-time effects of these two strains against P. solani, as indicated by the Pearson's chi-squared test and Hosmer-Lemeshow test. The dosage effect enhanced with the increase in infection time, or vice versa. The LC₅₀ of F061 was 5.55×10⁵ conidia/mL after an ten-day infection, and the LT₅₀ of 1.0×10⁸ conidia/mL was 5.04 d, both higher than those of FM-03 (1.11×10⁵ conidia/mL and 4.67 d), indicating higher virulence of FM-03 than F061. In addition, the laboratory contro1 efficiency of these two strains also increased with the increase in infection time, being 74.06% and 81.32% for 1×10⁸ conidia/mL of F061 and FM-03 in ten days, respectively. In conclusion, FM-03 can be preferentially used to control P. solani on succulent plants, while F061 can serve as an alternative strain.

Key words: Metarhizium anisopliae, Phenacoccus solani, laboratory virulence, laboratory contro1 efficiency

CLC Number:

S433
S476.1

HUANG Peng, CHEN Hanxin, YAO Jinai, HUANG Jiancheng, YU Deyi. Laboratory Assessment of Pathogenicity of Metarhizium anisopliae against Phenacoccus solani[J]. Chinese Journal Of Biological Control, 2019, 35(6): 884-890.

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