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Chinese Journal of Biological Control ›› 2021, Vol. 37 ›› Issue (4): 785-795.DOI: 10.16409/j.cnki.2095-039x.2021.04.006

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Control Effect of Bacillus subtilis Qh-618 on Oat Leaf Spot

YE Fuchun, MA Wenlin, YANG Xiaolong   

  1. Qinghai Institute of Animal Husbandry and Veterinary Technology, Xining 812100, China
  • Received:2020-09-04 Published:2021-09-18

Abstract: Oats leaf spot, caused by Drechslera avenae(Eidam) Shoemaker, is one of the most important diseases of oats (Avena sativa). It commonly happens in the filling stage, penetrates from leaves and leaf sheaths, and finally leads to great loss of oats yield. To control the disease effectively, a biocontrol strain Qh-618 was isolated from oat habitat by antagonistic analysis and hydrolase activity test. Qh-618 showed strong antagonistic activity against many pathogenic fungi, with the inhibition rate of more than 60% on mycelium growth and significant inhibition on spore germination D. avenae. In addition, Qh-618 was proved to have apparent protease, galactosidase, ferritase and indoleacetic acid production activities. Through physiological and biochemical analysis and 16S rDNA and gyrB gene sequence analysis, Qh-618 was identified as Bacillus subtilis. Greenhouse experiment showed that Qh-618 could controlthe oats leaf spot efficiently, with the efficiency of more than 65%, and meanwhile, promote the growth of oats. The chlorophyll content, root length, seedling length, root weight, seedling weight and other biomass of oats were all up-regulated with the treatment of Qh-618. Field experiment showed that Qh-618 could control oat leaf spot by 58.57%, and increased the chlorophyll content, plant height and biomass of oats significantly, which led to the increase of the yield by 11.2%. In conclusion, B. subtilis Qh-618 is a biocontrol agent with growth-promotion activity and high potential for controlling oat leaf spot.

Key words: oats, Drechslera avenae (Eidam) Shoemaker, Bacillus subtilis, biological control, plant growth promotion

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