Effects of Different Carbon and Nitrogen Sources on the Production of Antifungal Lipopeptides from Bacillus subtilis BAB-1

doi:10.16409/j.cnki.2095-039x.2021.02.007

Abstract

Abstract: Bacillus subtilis BAB-1 showed strong antifungal activity against the airborne diseases of facility vegetables. Previous studies have shown that lipopeptides were one of the major antifungal compounds produced by B. subtilis BAB-1. In order to increase the yield of lipopeptides produced by strain BAB-1, the effects of five carbon sources and five nitrogen sources on the yield of lipopeptides were analyzed by FPLC. The characteristics of crude lipopeptides were assayed by hemolysis test and oil spreading technique. The results showed that arbutin was the best carbon source for production of surfactin, with the yield of 2.95 times, 7.01 time, 4.26 times and 5.60 times as many as that of glucose, D-xylose, D-ribose and L-arabinose, respectively, and the crude lipopeptides of strain BAB-1 showed strongest hemolytic activity and oil discharge capacity. Arbutin and glucose were beneficial to production of fengycin, without significant difference in fengycin yield between them, but significantly higher than that of D-xylose, D-ribose and L-arabinose. L-asparagine and L-sodium glutamate were good nitrogen sources for production of antifungal lipopeptides, and there were no significant difference in the yield of surfactin and fengycin between them, while the yield of antifungal lipopeptides were significantly higher than that of L-cysteine, L-glutamine and L-proline. When L-asparagine and L-sodium glutamate were used as nitrogen sources, the crude lipopeptide of strain BAB-1 showed stronger hemolytic activity and oil discharge capacity. The effects of five carbon substances on the expression of surfactin synthetase gene-srfAA were analyzed by quantitative reverse transcription PCR (RT-qPCR). The results showed that the gene-srfAA was positively affected by arbutin and the expression of srfAA was improved about 2.9 times compared to that of glucose. The above results provided theoretical support for the optimization of the fermentation of strain BAB-1 for production of lipopeptides and its lagre-scale industrial production.

Key words: Bacillus subtilis, lipopeptides, carbon source, nitrogen source

CLC Number:

S476

ZHANG Xiaoyun, GUO Qinggang, WANG Peipei, SU Zhenhe, LU Xiuyun, ZHAO Weisong, QU Yuanhang, DONG Lihong, CONG Rong, LI Shezeng, MA Ping. Effects of Different Carbon and Nitrogen Sources on the Production of Antifungal Lipopeptides from Bacillus subtilis BAB-1[J]. Chinese Journal of Biological Control, 2021, 37(2): 251-258.

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