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Chinese Journal of Biological Control ›› 2021, Vol. 37 ›› Issue (4): 761-770.DOI: 10.16409/j.cnki.2095-039x.2021.04.005

• RESEARCH REPORTS • Previous Articles     Next Articles

Identification of Antagonistic Bacterium Bacillus velezensis Hsg1949 and Its Biocontrol Effect on Peanut Pod Rot

CAO Weiping1, LU Qing2, LU Xiuyun1, ZANG Weiping3, SONG Jian1   

  1. 1. Institute of Plant Protection, Hebei Academy of Agricultural and Forestry Sciences/IPM Center of Hebei Province/Key Laboratory of Integrated Pest Management on Crops in Northern Region of North China, Ministry of Agriculture, Baoding 071000, China;
    2. Tangshan Academy of Agricultural Sciences, Tangshan 063000, China;
    3. Baoding Longtan Park Management Office, Baoding 071000, China
  • Received:2020-12-21 Published:2021-09-18

Abstract: Pod rot caused by complex infections of a variety of pathogens leads to destructive losses of yield and quality of peanut. To obtain the biocontrol microorganism for controlling pod rot of peanut, an antagonistic bacterial strain Hsg1949 was screened from the rhizosphere soil of peanut by agar disk dilution and dual-culture assay using pathogenic fungi Fusarium spp. as indicators. Based on morphological characters, biolog identification system in combination with 16S rDNA and gyrA sequence analysis, the strain Hsg1949 was identified as Bacillus velezensis. After 40 generations of cultivation, Hsg1949 showed stable antifungal activity against three Fusarium spp. isolates, and the inhibition rates were 77.0%, 69.7 and 61.9%, respectively, without significant difference to the original generation. The cell-free supernatant also showed stable inhibitory effects against Fusarium spp. under high temperature and strong acid, however, the inhibitory activities decreased significantly when the pH value of supernatant was 11. Field trials showed that the biocontrol efficacies of Hsg1949 were 66.54% and 71.27% at concentrations of 2×108 CFU/mL and 5×108 CFU/mL, respectively, similar to that of carbendazim (75.11%). Also, the strain Hsg1949 exhibited antagonistic activity against four plant pathogenic fungi, including Sclerotium rolfsii, Rhizoctonia solani, Fusarium oxysporum and Verticillium dahliae. In summary, the strain Hsg1949 has excellent biocontrol characteristics and potential in biocontrol of plant diseases.

Key words: pod rot of peanut, Fusarium spp., Bacillus velezensis, antagonistic activity, biocontrol effect

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