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Chinese Journal of Biological Control ›› 2022, Vol. 38 ›› Issue (5): 1156-1165.DOI: 10.16409/j.cnki.2095-039x.2021.11.004

• RESEARCH REPORTS • Previous Articles     Next Articles

Screening for qRT-PCR Reference Genes in Trichogramma japonicum

WANG Shanshan1,2, TIAN Junce1,2, LU Yanhui2, XU Hongxing1, WANG Xiangping1, LÜ Zhongxian2   

  1. 1. Forewarning and Management of Agricultural and Forestry Pests, Hubei Engineering Technology Center, Yangtze University, Jingzhou 434025, China;
    2. State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products/Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
  • Received:2021-08-21 Published:2022-10-20

Abstract: For screening suitable reference genes used in the studies of Trichogramma japonicum,ten candidate reference genes (including PABPC1L, ACTR2, ARPC2, EF1a, EEF1D, SFRS7, HSP70, CSNK1a1, GAPDH, TMEM209),selected on the base of T.japonicum transcriptome data,were evaluated for the expression stability under different conditions such as temperatures,diets,insecticides and developmental duration.The stabilities were analyzed by Delta CT,BestKeeper,NormFinder and GeNorm and finally sorted by RefFinder.The results indicated that the genes EF1a,EEF1D,SFRS7,ARPC2,PABPC1L,TMEM209,PABPC1L and ARPC2 were expressed the most stable at different temperatures,when fed with different diets,under different insecticide treatments,and at different developmental durations,respectively.The results of this study can help select reference genes in T.japonicum under different conditions,and also help obtain more reliable and accurate data for gene expression in T.japonicum.

Key words: qRT-PCR, Trichogramma japonicum, reference gene, gene expression

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