Optimization of Solid-State Fermentation Process of Penicillium simplicissimum CEF-818

doi:10.16409/j.cnki.2095-039x.2022.02.026

Abstract

Abstract: The fermentation product of Penicillium simplicissimum CEF-818 can effectively control cotton Verticillium wilt caused by Verticillium dahliae. In order to optimize fermentation conditions of P. simplicissimum CEF-818, the composition of solid fermentation medium and fermentation conditions of CEF-818 were screened by single factor and orthogonal test. The results showed that the optimum culture for solid fermentation of P. simplicissimum CEF-818 was wheat bran, and addition of 2.5% glucose (M/M), 0.6% urea (M/M) and 0.6% potassium chloride (M/M), with the initial pH value of 7.0, the ratio of material to water 1:0.5, the liquid inoculums level of 5% (V/M), the material thickness of 2 cm and at 28℃ for 7 days. Under this optimal medium and fermentation process, the sporulation could reach to 8.28×10⁹ CFU/g for P. simplicissimum CEF-818.

Key words: biocontrol, Penicillium simplicissimum, solid fermentation, sporulation

CLC Number:

S476

PU Dandan, LI Yan, ZHAO Lihong, ZHANG Yalin, WEI Feng, FENG Hongjie, ZHU Heqin, GU Aixing, PENG Jun, FENG Zili. Optimization of Solid-State Fermentation Process of Penicillium simplicissimum CEF-818[J]. Chinese Journal of Biological Control, 2022, 38(3): 636-644.

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