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Chinese Journal of Biological Control ›› 2022, Vol. 38 ›› Issue (1): 115-124.DOI: 10.16409/j.cnki.2095-039x.2021.06.012

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Screening of Antagonistic Trichoderma against Cassava Anthracnose and Investigation on Its Control Effect in Laboratory

ZHANG Jingya1, LI Xinyu1, ZHANG Cheng1, WANG Weiwei2, ZHANG Peng1, HOU Jumei2, LIU Tong1,2   

  1. 1. Plant Protection College of Hainan University/Key Laboratory of Green Prevention and Control of Tropical Agroforestry Biological Disasters, Ministry of Education, Haikou 570228, China;
    2. Engineering Center of Agricultural Microbial Preparation Research and Development of Hainan(Hainan University), Haikou 570228, China
  • Received:2021-02-23 Online:2022-02-08 Published:2022-03-01

Abstract: The pathogen of cassava anthracnose was identified, and its antagonistic Trichoderma isolates were screened and identified. Through the method of tissue separation and Koch's rule, the pathogen of cassava anthracnose was identified as Colletotrichum gloeosporioides. Three Trichoderma strains (LG004-52, LS073-23 and ZJB3-12) showing strong inhibitory effect against C. gloeosporioides were screened from 15 strains of Trichoderma strains, with inhibition rates of 82.7%, 82.7% and 83.5%, respectively. Through the antimicrobial test of volatiles and fermentation broth, it was found that the inhibition ratios of volatiles produced by the three Trichoderma strains were all more than 50% and inhibition rate of the fermentation broth produced by Trichoderma ZJB3-12 reached to 100%. Based on morphological observation and phylogenetic analysis of rpb2 gene, LG004-52, LS073-23 and ZJB3-12 were identified as T. breve, T. asperellum and T. longibrachiatum, respectively. Indoor control effect test showed that the Trichoderma ZJB3-12 could strongly inhibit the lesion formation of cassava anthracnose disease, and the inhibition rate was 68.5% compared with the control. These results showed that T. longibrachiatum ZJB3-12 has the potential to be developed as bioctmtrol agent for cassava anthracnose control.

Key words: cassava anthracnose, Trichoderma spp., volatile substances, fermented broths, biocontrol

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