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Chinese Journal Of Biological Control ›› 2019, Vol. 35 ›› Issue (6): 940-948.DOI: 10.16409/j.cnki.2095-039x.2019.06.022

• RESEARCH REPORTS • Previous Articles     Next Articles

Screening and Identification of Pseudomonas against Tobacco Black Shank and Tobacco Root Black Rot

QIAN Huimin1, WEN Yi1, ZHAO Hui1, NI Yunxia1, LIU Xintao1, QIU Rui2, LI Xiaojie2, ZHAO Xinbei1, LI Shujun2, LIU Hongyan1   

  1. 1. Institute of Plant Protection, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China;
    2. Key Laboratory for Green Preservation&Control of Tobacco Diseases and Pests in Huanghuai Growing Area/Tobacco Research Institute of Henan Academy of Agricultural Sciences, Zhengzhou 450002, China
  • Received:2019-04-02 Online:2019-12-08 Published:2019-12-18

Abstract: In order to obtain Pseudomonas bacteria with good biocontrol effect on tobacco black shank disease and black root rot disease, which can also promote tobacco growth, 201 biocontrol bacteria were isolated from 40 soil samples. Using plate confrontation method and poison media method, we ultimately obtained bacteria strains PA2101 and PG3402 that had stronger antagonistic effect on the pathogens of tobacco black shank disease and black root rot disease. The pot experiment showed that PA2101 and PG3402 could improve the growth of aboveground plants and root system in a coordinated way. Both of them could increase the plant height, leaf area, aerial fresh weight, root fresh weight, leaf number and root length of tobacco, as well as the root shoot ratio and root activity of tobacco. The control efficacies of PA2101 on tobacco black shank and black root rot were 70.11% and 62.67%, which were better than their fungicide control. And the control efficacies of PG3402 on the two tobacco diseases were 60.92% and 60.00%, which were equivalent to that of fungicide control. Colonization test with resistant label method showed that PA2101 and PG3402 could stably colonize on tobacco rhizosphere soil and root on 29th day after inoculation, and that they could be detected in tobacco stem and leaf, indicating that the two strains could well colonize. On the basis of molecular analysis of 16S rDNA sequences, colonial morphology, as well as physiological and biochemical characteristics, PA2101 and PG3402 were identified as Pseudomonas aeruginosa and Pseudomonas granadensis respectively. PA2101 and PG3402 have good growth-promoting effects and disease prevention effects on tobacco black shank disease and root black rot disease, indicating that they are of great potential in the biocontrol on the two tobacco fungal diseases.

Key words: tobacco black shank, tobacco black root rot, biocontrol, Pseudomonas sp., identification

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