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Chinese Journal of Biological Control ›› 2022, Vol. 38 ›› Issue (5): 1075-1082.DOI: 10.16409/j.cnki.2095-039x.2022.09.003


The Effects of Silencing of HvFTZ-F1 via RNAi on the Survival and Development of the 28-Spotted Potato Ladybird,Henosepilachna vigintioctopunctata Larvae

WANG Yajie1, LIU Zhuoqi1, LI Huali2, PAN Huipeng1, YANG Chunxiao2   

  1. 1. College of Plant Protection, South China Agricultural University/Key Laboratory of Bio-Pesticide Innovation and Application of Guangdong Province, Guangzhou 510642, China;
    2. South China Agricultural University, State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangzhou 510642, China
  • Received:2022-07-13 Published:2022-10-20

Abstract: Henosepilachna vigintioctopunctata is a major pest of Solanaceae plants,causing serious economic losses to crop production in China.RNAi (RNA inteference) has been found as a novel management strategy for controlling this pest.FTZ-F1 is a key regulator of insect growth and development.In this study,by feeding 5 ng/μL dsHvFTZ-F1 solution treated eggplant leaves to 1st instar larvae of H.vigintioctopunctata,the expression level of HvFTZ-F1 was significantly reduced by 63.69%,the larvae were unable to molt normally into the 2nd instar,with the mortality as high as 65.00%.Meanwhile,silencing of HvFTZ-F1 caused the expression levels of 20E related genes,HvDIB,HvSPOOK,HvSHADOW,HvSHADE,and HvE75 was significantly reduced by 85.36%,66.32%,52.74%,31.12%,and 20.76%,respectively;the expression levels of pigmentation-related genes,HvDDC,HvTH,and Hvebony was significantly reduced by 88.74%,91.97%,and 86.15%,respectively;20E titer and dopamine titer decreased by 15.97% and 21.38%,respectively.In summary,HvFTZ-F1 plays an important role in the survival and development of H.vigintioctopunctata.HvFTZ-F1 can be used as an efficient target gene for the development of RNA biopesticide based on RNAi technology for controlling H.vigintioctopunctata.

Key words: Henosepilachna vigintioctopunctata, HvFTZ-F1, RNAi, 20E titer, dopamine titer

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