Evaluation of the Potential Allergenicity of Icarapin-like Proteins of Two Pteromalid Wasps

doi:10.16409/j.cnki.2095-039x.2022.11.017

Abstract

Abstract: The icarapin protein of Western honey bee, Apis mellifera, is a common human allergen and its homologues also exist in parasitoid wasps. In order to evaluate the potential allergenicity of icarapin-like proteins in parasitoid wasps, the icarapin-like protein encoding genes from Pteromalus puparum and Nasonia vitripennis were successfully cloned and expressed. Sequence analysis showed that the open reading frames of icarapin-like genes in the two parasitoid wasps were 654 bp and 714 bp, respectively, and both were predicted to be potentially allergenic. The recombinant icarapin-like proteins were purified by Ni-ion affinity chromatography. Using the BALB/c mouse model for potential allergenicity assessment, we found that the concentrations of histamine and immunoglobulin E (IgE) in mice serum injected with recombinant proteins of the two parasitoid wasps and A. mellifera were higher than those of the negative control group injected with PBS, indicating that icarapin-like proteins from the two pteromalid wasps have potential allergenicity. This study provides a reference for the pre-application safety assessment of parasitoid bioactive proteins.

Key words: Pteromalus puparum, Nasonia vitripennis, icarapin-like protein, expression and purification, allergenicity assessment

CLC Number:

S476.3

CAO Yunshen, YANG Yi, FANG Qi, WANG Fang, YE Gongyin. Evaluation of the Potential Allergenicity of Icarapin-like Proteins of Two Pteromalid Wasps[J]. Chinese Journal of Biological Control, 2023, 39(5): 1049-1057.

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