Effects of Pollen-added Artificial Diets on the Development of Harmonia axyridis Larvae

doi:10.16409/j.cnki.2095-039x.2022.11.019

Abstract

Abstract: Effective artificial diet is important for mass-rearing of predatory ladybird beetles. However, most insect-free artificial diets generally result in inferior rearing efficiency. Plant pollens are rich in nutritive and non-nutritive components and are important alternative diets for the predatory ladybird beetles under natural conditions. Until now, there are scarcely reports that pollen can be applied as a component of artificial diet. In this study, maize pollen and rose pollen were separately supplemented at three concentrations into the basic artificial diet (Control) of Harmonia axyridis, and then used to rear the 1^st instar larvae the predator. The aim of this study is to evaluate the potential of pollen in improving diet quality and the impact of pollen sources and concentrations. The results showed that, when fed with pollen-added artificial diets, the H. axyridis 1^st instars survived to adults at 68.33%―83.33%, which was significantly higher than that obtained with control diet (36.67%); however the total developmental duration and body weights at adult emergence were not significantly different. Pollen sources and concentrations greatly affected the development of H. axyridis larvae. Feeding on high concentration rose pollen (RS-H) and median concentration maize pollen-amended artificial diets (MZ-M) resulted in the highest survival rates of 1^st instar-to-adult (83.33% and 80.00%, respectively). Our results indicate that supplementation of pollen can greatly improve the diet quality, and RS-H and MZ-M have great potential being used in mass-rearing of H. axyridis larvae.

Key words: pollen, Harmonia axyridis, artificial diet, mass-rearing, survival rate

CLC Number:

S476.2

SUN Yuanxing, CHEN Mingjuan, LI Mingling, QIN Yalong, ZHONG Mingjuan. Effects of Pollen-added Artificial Diets on the Development of Harmonia axyridis Larvae[J]. Chinese Journal of Biological Control, 2023, 39(5): 1058-1065.

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