Identification and Expression Analysis of spo0E Gene Family in Brevibacillus brevis Strain B011

doi:10.16409/j.cnki.2095-039x.2023.02.033

Abstract

Abstract: Brevibacillus brevis B011 is an endophytic bacteria, which was isolated from the healthy tobacco root, and could produce endospores at the late stages of fermentation. According to reported results, the product of the Spo0E gene can dephosphorylate Spo0A~P and then regulates spore formation negatively. In order to further verify the function and expression of the Spo0E gene in the B011 genome and provide candidate genes for genetic modification of the strain, we identified 9 spo0E-like genes in the whole genome sequences of strain B011 using bioinformatics tools. The spo0E gene families in Brevibacillus were differentiated before the separation of species by comparing the whole genome of 101 strains of Brevibacillus. Two spo0E-like genes, FO446_19435 and FO446_04050, were abundantly expressed in B011 and both of them increased first, then decreased and the expression level reached the highest peak in the broth after 18 h. Both of these two spo0E-like genes contained SQELD motif and we proposed they are the two main spo0E genes in strain B011.

Key words: Brevibacillus brevis, endospore, whole genome sequences, spo0E gene

CLC Number:

Q939.96

ZHOU Xiangping, WANG Yunsheng, CHEN Wu, LIU Tianbo, WANG Kaige, LIU Feng, ZHOU Li, YUAN Zhihui. Identification and Expression Analysis of spo0E Gene Family in Brevibacillus brevis Strain B011[J]. Chinese Journal of Biological Control, 2023, 39(4): 895-903.

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