Medium Optimization for the Production of Antimicrobial Ethylparaben from Brevibacillus brevis FJAT-0809-GLX using Response Surface Methodology

doi:10.16409/j.cnki.2095-039x.2017.02.016

Abstract

Abstract: Ethylparaben is the main antimicrobial substance of Brevibacillus brevis FJAT-0809-GLX. To improve the yield of ethylparaben, the fermentation medium components were optimized using response surface method. The carbon sources, nitrogen sources and inorganic salts in the fermentation medium were optimized by single factor experiment. Plackett-Burman design was undertaken to evaluate the influence factors of the culture medium. The path of steepest ascent was adopted to approach the optimal region of the three significant factors, and then Box-Behnken design and response surface methodology were applied to determine the optimal levels of the main factors. The results showed that the best carbon source, nitrogen source and inorganic salt were DL-malic acid, peptone and NaCl, respectively; DL-malic acid, soybean cake powder and NaCl were the main factors of the culture medium; The best fermentation medium for ethylparaben production of this strain contained soluble starch 8 g/L, DL-malic acid 29.68 g/L, bean powder 25.18 g/L, peptone 2 g/L, NaCl 13.18 g/L and pH 7.0-7.2. Using this fermentation medium, the average yield of ethylparaben was increased by 286.26% compared the basal fermentation medium and reached up to 8.15 μg/mL.

Key words: Brevibacillus brevis, ethylparaben, response surface method, DL-malic acid

CLC Number:

S476

CHE Jianmei, LIU Bo, CHEN Bingbing, LIU Guohong, GE Cibin, LAN Jianglin. Medium Optimization for the Production of Antimicrobial Ethylparaben from Brevibacillus brevis FJAT-0809-GLX using Response Surface Methodology[J]. journal1, 2017, 33(2/3): 248-257.

References

[1] Cook R J, Bruckart W L, Coulson J R, et al. Safety of microorganisms intended for pest and plant disease control:a frame work for scientific evaluation[J]. Biological Control, 1996, 7(3):333-351.
[2] Panwar S, Singh L, Sunaina V. Evaluation of Brevibacillus brevis and Bacillus firmusstrains in management of fungal pathogens[J]. Pesticide Research Journal, 2000, 18(2):159-161.
[3] 车建美, 刘波, 郭慧慧, 等. 短短芽胞杆菌FJAT-0809-GLX对番茄促长作用的研究[J]. 福建农业学报, 2015, 30(5):498-503.
[4] Che J M, Liu B, Ruan C Q, et al. Biocontrol of Lasiodiplodia theobromae, which causes black spot disease of harvested wax apple fruit, using a strain of Brevibacillus brevis FJAT-0809-GLX[J]. Crop Protection, 2015, 67(1):178-183.
[5] 车建美, 付萍, 刘波, 等. 保鲜功能微生物FJAT-0809-GLX对龙眼保鲜特性的研究[J]. 热带作物学报, 2010, 31(9):1632-1640.
[6] Archna G, Kaushik C P, Kaushik A. Degradation of hexachlorocyclohexane (HCH; α, β, γ and δ) by Bacillus circulans and Bacillus brevis isolated from soil[J]. Soil Biology and Biochemistry, 2000, 32(11/12):1803-1805.
[7] Arutchelvan V, Kanakasabai V, Elangovan R, et al. Kinetics of high strength phenol degradation using Bacillus brevis[J]. Journal of Hazardous Materials B, 2006, 129(1-3):216-222.
[8] Jaouadi N Z, Rekik H, Badis A, et al. Biochemical and molecular characterization of a serine keratinase from Brevibacillus brevis US575 with promising keratin-biodegradation and hide-dehairing activities[J]. PLoS ONE, 2013, 11(8):e76722.
[9] Wang J, Haddad N, Yang S Z, et al. Structural characterization of lipopeptides from Brevibacillus brevisHOB1[J]. Applied Biochemistry and Biotechnology, 2009, 160(3):812-821.
[10] 郝晓娟, 刘波, 谢关林, 等. 短短芽孢杆菌JK-2菌株抑菌物质特性的研究[J]. 浙江大学学报(农业与生命科学版), 2007, 33(5):484-489.
[11] 杨廷雅, 孙亮, 周婷婷, 等. 短短芽孢杆菌Brevibacillus brevis HAB-5主要抑菌活性成分的分析及其特性研究[J]. 中国生物防治学报, 2014, 30(2):222-231.
[12] Che J M, Liu B, Chen Z,et al. Identification of ethylparaben as the antimicrobial substance produced by Brevibacillus brevis FJAT-0809-GLX[J]. Microbiological Research, 2015, 172(1):48-56.
[13] 车建美, 刘波, 陈冰冰, 等. 一株短短芽胞杆菌中羟苯乙酯高效液相色谱分析研究[J]. 农业生物技术学报, 2015, 23(11):1524-1530.
[14] 胡桂萍, 刘波, 朱育菁, 等. 少动鞘脂单胞菌产结冷胶发酵培养基的响应面法优化[J]. 生物数学学报, 2012, 27(3):507-517.
[15] 邹娟, 黄悦, 姚蓉, 等. 响应面法优化芽孢杆菌B-6产絮凝剂的发酵培养基[J]. 生物数学学报, 2014, 29(3):538-546.
[16] 夏尚远. 1株短短芽孢杆菌产抗菌物质发酵培养基的优化[J]. 中国兽医杂志, 2015, 51(7):91-93.
[17] 芮广虎, 胡雪芹, 殷坤, 等. 响应面法优化短短芽孢杆菌FM4B的发酵培养基[J]. 食品科学, 2012, 33(15):257-261.
[18] 陈凯, 薛东红, 夏尚远, 等. 短短芽孢杆菌Brevibacillus brevisXDH菌株发酵培养基配方的研究[J]. 山东农业大学学报(自然科学版), 2006, 37(2):190-195.
[19] 郝晓娟, 刘波, 葛慈斌, 等. 短短芽孢杆菌JK-2菌株抑菌活性物质产生条件的优化[J]. 河北农业科学, 2009, 13(6):46-49.
[20] 陈峥, 刘波, 朱育菁, 等. pH条件对短短芽孢杆菌FJAT-0809-GLX次生代谢物产生的影响[J]. 福建农业学报, 2012, 27(1):71-76.
[21] 刘辉, 魏祥法, 井庆川, 等. 一株枯草芽孢杆菌发酵培养基的优化[J]. 山东农业科学, 2007(1):100-101.
[22] 卢行, 李荣, 肖启明, 等. 一株短短芽孢杆菌X23培养基优化及其抑菌活性研究[J]. 湖南农业科学, 2014(11):1-2.
[23] 林司曦, 吴小芹, 丁晓磊, 等. 短小芽胞杆菌Bacillus pumilus HR10增殖扩繁培养基组分优化[J]. 微生物学杂志, 2014, 34(6):22-28.
[24] 张蕾, 石新丽, 李敏, 等. 短小芽孢杆菌ty079产抗菌物质的发酵培养基研究[J]. 河北省科学院学报, 2010, 27(2):47-50.
[25] 顾小平, 吴晓丽. 毛竹根际分离的多粘芽孢杆菌固氮特性的研究[J]. 林业科学研究, 1998, 11(4):377-381.