Welcome to Chinese Journal of Biological Control,Today is

Chinese Journal of Biological Control ›› 2023, Vol. 39 ›› Issue (5): 1156-1171.DOI: 10.16409/j.cnki.2095-039x.2023.02.055

• RESEARCH REPORTS • Previous Articles     Next Articles

Analysis of Inhibitiory Active Substances of Penicillium bilaiae Strain 47M-1 Based on Non-Targeted Metabolomics

ZHAO Xinbei, NI Yunxia, LIU Xintao, ZHAO Hui, YAN Wenqing, HE Bipo, LIU Hongyan   

  1. Institute of Plant Protection, Henan Academy of Agricultural Sciences/Key Laboratory of Integrated Pest Management on Crops in Southern Region of North China, Ministry of Agriculture and Rural Affairs/Henan Key Laboratory of Crop Pest Control/Biological Pesticides Engineering Research Center of Henan Province/International Joint Research Laboratory for Crop Protection of Henan/Agricultural Microbiology Innovation Center of Henan Province, Zhengzhou 450002, China
  • Received:2023-03-28 Online:2023-10-08 Published:2023-10-25

Abstract: Strain 47M-1 of Penicillium bilaiae showed significant inhibitory effect on a variety of sesame pathogenic fungi. In order to analyze the inhibitiory active substances produced by this strain, liquid chromatography tandem mass spectrometry was used to detect the metabolites of strain cultured for 48 h, 96 h and 144 h. Then, classification of up-regulated differential metabolites in the HMDB database and enrichment of KEGG metabolic pathways were analyzed in order to identify the active metabolites. A total of 105 up-regulated differential metabolites were detected, which were mainly enriched in the superclasses of phenylpropanoids and polyketides. The enrichment analysis of the KEGG metabolic pathways showed that the up-regulated differential metabolites were mainly enriched in metabolic pathways, biosynthesis of cofactors, purine metabolism, biosynthesis of amino acids and ABC transporters. The inhibitiory activities of some up-regulated differential metabolites were verified using medicated plate method. These compounds naringenin, protocatechuic acid, D-(-)-quinic acid, hesperetin, 3-(3-Hydroxyphenyl) propanoic acid, 3, 4-dihydroxyhydrocinnamic acid, 5, 7-dihydroxy-2-(4-hydroxyphenyl)-3, 4-dihydro-2H-1-benzopyran-4-one, rosmarinic acid, bergapten and eriodictyol all showed excellent inhibition activities against sesame pathogenic fungi. In this study, the major groups and key metabolic pathways of potential inhibition metabolites produced by strain 47M-1 were identified, and the inhibitiory activity of some up-regulated differential metabolites was verified. This study will provide data support for the further development and utilization of strain 47M-1 and the research and development of microbial pesticide.

Key words: Penicillium bilaiae, metabolome, inhibition active substances, phenylpropanoids and polyketides, metabolic pathways

CLC Number: