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Chinese Journal of Biological Control ›› 2023, Vol. 39 ›› Issue (6): 1373-1383.DOI: 10.16409/j.cnki.2095-039x.2023.02.065

• RESEARCH REPORTS • Previous Articles     Next Articles

Preparation and Process Optimization of Feather Carbon-based Bilayer Microcapsules of Paecilomyces lilacinus M-1

XIE Xiaolin1, ZHOU Lian1, CHEN Meng3, LI Chengjiang1, WANG Yong2, LIU Yumin1, LI Yanxuan1, YAO Qing3, ZHU Honghui1   

  1. 1. Guangdong Institute of Microbiology/Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application/Guangzhou Provincial Open Laboratory of Microbial New Application Technique/State Key Laboratory of Applied Microbiology, South China (The Ministry-Province Joint Development), Guangzhou 510070, China;
    Guangdong Bowote Biotechnology Co., LTD, Shaoguan 512000, China;
    3. South China Agricultural University, College of Agriculture, Guangzhou 510642, China
  • Received:2023-06-09 Online:2023-12-08 Published:2023-12-20

Abstract: Paecilomyces lilacinus has shown great potential in biological control of nematodes. However, its large-scale market application is limited by short product shelf life, low soil colonization rate, and unstable biocontrol effect. To solve these problems, this study developed a spray method combined with calcium ion solidification method to establish the best embedding process for P. lilacinus M-1 microcapsules. The optimal process conditions for preparing P. lilacinus M-1 microcapsules were determined by single-factor and response surface experiments through optimization. The best process parameters were: sodium alginate addition 3.183%, mass ratio of spores to sodium alginate solution 1.71:100, expanded feather powder addition 4%, activated carbon addition 4%, feed flow rate 3.7 L/h, CaCl2 concentration 3%, chitosan concentration 0.858%. Under this optimized condition, the stability of microcapsule strains in different environments was better than that of powder strains. Microcapsules are stored at room temperature for 12 months and still retain more than 20% of the total viable count. Soil colonization experiments showed that the soil colonization effects of different P. lilacinus M-1 formulations were as follows: feather carbon-based bilayer microcapsules > carbon-based bilayer microcapsules > powder. Therefore, this process solves the bottleneck problem of large-scale market application of P. lilacinus and has high application value.

Key words: P. lilacinus, microcapsule, root-knot nematode, colonization rate, response surface experiments

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