Control Efficacy of Ochrobactrum pseudogrignonese A-29 against Southern Root-knot Nematode and Its Identification

doi:10.16409/j.cnki.2095-039x.2022.03.008

Abstract

Abstract: The southern root-knot nematode, Meloidogyne incognita is one of the most damaging plant-parasitic nematodes worldwide, and it can cause severe economic losses to agriculture. In this study, the antagonistic activity of strain A-29 fermentation broth against M. incognita was evaluated in vitro and in pot experiment. Furthermore, the taxonomic species of strain A-29 was identified. Four treatments were applied on second-stage juveniles (J2s) and eggs as follows: 1 x, 5 x, 10 x dilution of the fermentation filtrate of strain A-29 and a control. The fermentation filtrate of the strain A-29 showed significant potential nematicidal activities on J2s, and the remarkable inhibitory effects on egg hatching. The mortality of J2s was ranged from 71.09% to 95.45% after 24 h and 48 h; and the egg hatching inhibition was 59.25% to 87.86% after 4 d. Furthermore, the mortality percentage of J2 and eggs increased as the increase of fermentation concentration. After 60 days, the tomato seedlings with A-29 fermentation broth treatment in the pot experiment, the control efficacy was up to 75.00%, and the whole fresh weight, root dry weight and height of tomato plants were increased by 38.41%, 33.52% and 20.01%, respectively, when compared with the control. In addition, the taxonomic status of the strain A-29 was investigated and identified as Ochrobactrum pseudogrignonese based on morphological, physiological and biochemical characteristics, 16S rRNA gene sequence and Bayesian phylogenetic analysis. The results showed that O. pseudogrignonese strain A-29 exhibited high antagonistic activity against M. incognita in vitro and pot experiments, and can increase the tomato plant biomass. It is suggesting that O. pseudogrignonese strain A-29 could be considered as a potentially new biological nematicide for the control of southern root-knot nematode.

Key words: southern root-knot nematode, Ochrobactrum pseudogrignonese, control efficacy

CLC Number:

S476.2

YAO Yanan, FENG Yeqing, GENG Jingjing, XU Yumei, ZHAO Zengqi, WANG Jianming. Control Efficacy of Ochrobactrum pseudogrignonese A-29 against Southern Root-knot Nematode and Its Identification[J]. Chinese Journal of Biological Control, 2022, 38(2): 367-373.

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