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Chinese Journal Of Biological Control ›› 2020, Vol. 36 ›› Issue (4): 596-603.DOI: 10.16409/j.cnki.2095-039x.2020.04.021

• RESEARCH REPORTS • Previous Articles     Next Articles

Microcapsule Preparation for Trichoderma asperellum Tr266B

YU Wenqian, WANG Chengfang, KUANG Wenfeng, CHEN Chen, MAO Weili   

  1. Shanghai W. L. H. -Biotech Corporation, Shanghai 201203, China
  • Received:2019-12-10 Online:2020-08-08 Published:2020-08-07

Abstract: A spray drying technology was developed and utilized for Trichoderma microcapsule preparation. By use of single factor and orthogonal tests, the technical parameters of spray drying and microcapsule formulation were optimized. The inlet and outlet air temperatures (T-in, T-out) were (92±2)℃ and (55±2)℃, respectively. The formula of microcapsule was as follows:the solid content of condensed liquid of fermented T. asperellum (Tr266B) prepared for spray was 20%; the contents of YHA and maltodextrin were 15%, respectively, and the contents of arabic gum and glycerin were 2%, respectively. After spray drying, the water content of the microcapsule was 7.5%-8.5%. The effects of temperatures during spray drying and storage and the water content of microcapsule on spores germination rate were also investigated. The results showed that T-in and T-out had significant effects on spores germination rate during spray drying. If setting T-in and T-out higher than (95±2)℃ and (65±2)℃, respectively, the spores germination rate can be reduced 10%-40%. When the storage temperature was at 4℃ and the water content of microcapsule was 7.5%-8.5%, the spore germination rate did not change apparently after 6 months of storage. When the water content was less than 6.5%, the spores germination rate reduced gradually along with the storage time. However, the spores germination rate stored at 25℃ was less than 10% after 6 months. The wall materials of microcapsules were determined to be YHA, maltodextrin and arabic gum, and the humectant was glycerin. These materials have good biocompatibility with T. asperellum Tr266B, which will provide a reference for industrial mass production and product storage of Trichoderma microcapsules.

Key words: spray drying, microcapsule, Trichoderma spp., germination rate, water content

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