Olfactory Behavior Responses of Odontothrips loti Female Adults to Four Leguminous Plants and their Volatiles

doi:10.16409/j.cnki.2095-039x.2023.09.002

Abstract

Abstract: The olfactory behavior responses of the thrip Odontothrips loti female adults to their host plants, four leguminous forages, Medicago sativa, Melilotus officinalis, Onobrychis viciaefolia, and Trifolium pratense, were tested using 'Y' type olfactometer. The dynamic headspace sampling was used to collect plant volatiles, and gas chromatography-mass spectrometry (GC-MS) was used to identify the chemical composition and content of the volatiles. The results showed that female adults of O. loti significantly preferred flower odors of M. sativa with a selection rate of 70.6%, while showed no significant preference for the other three hosts, M. officinalis, O. viciaefolia and T. pratense. In dual-choice behavioral assays, O. loti females were significantly attracted to M. sativa in comparison with O. viciaefolia and T. pratense, with a selection rate of 78.3% and 68.7%, respectively. There were no significant differences in preference in other treatment combinations. A total of 40 volatile components, including 1 alkane, 9 alcohols, 2 ketones, 5 aldehydes, 9 esters, 13 terpenes, and 1 phenol, were identified from the volatiles. The factors 1, 2 and 3 extracted by principal component analysis explained 56.65%, 24.47% and 15.63% of the total variance, respectively. The contribution to the three factors to volatile components ranked from high to low was M. sativa, M. officinalis, O. viciaefolia, and T. pratense. The crucial host plant volatile components, such as benzaldehyde, β-Caryophyllene, (+)-α-pinene, myrcene, methyl palmitate, and thymol, probably play important roles in chemosensory communication between O. loti and its host plants. These results provide a basis for further development of plant-derived attractants for the green control of O. loti.

Key words: Odontothrips loti, Medicago sativa, volatile, olfactory

CLC Number:

S541

CUI Xiaoning, ZHAO Xiaodong, XI Boxin, HU Guixin, SHANG Suqin, PENG Bin, SHI Xueqiang. Olfactory Behavior Responses of Odontothrips loti Female Adults to Four Leguminous Plants and their Volatiles[J]. Chinese Journal of Biological Control, 2023, 39(4): 824-833.

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