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Chinese Journal of Biological Control ›› 2024, Vol. 40 ›› Issue (2): 435-447.DOI: 10.16409/j.cnki.2095-039x.2024.02.016

• RESEARCH REPORTS • Previous Articles    

Screening of Antagonistic Bacterium HB-10 Against Wheat Sheath Blight and Optimization of Fermentation Conditions

CHENG Liangliang, YE Lei, WANG Wenkai, TANG Jianlin, TAN Genjia   

  1. College of Plant Protection, Anhui Agriculturel University, Key Laboratory of Integrated Pest Management of Anhui Province Hefei 230036, China
  • Received:2023-03-28 Published:2024-04-28

Abstract: To develop biocontrol strains with preventive and therapeutic effects against wheat sheath blight,this study screening HB-10 strain.HB-10 strain exhibited significant control effects against the Rhizoct.onia cerealis.The strain was identified,optimized for fermentation,and evaluated through pot experiments to assess its efficacy.Results showed that HB-10 strain achieved an inhibition rate of 89.72% against Rhizoctonia cerealis.Morphological and molecular identification confirmed HB-10 strain as Pseudomonas aeruginosa.Antimicrobial spectrum analysis demonstrated the strong antagonistic effects of HB-10 strain against various pathogens,including Magnaporthe oryzae,Fusarium moniliforme,Alternaria alternata and Sclerotinia Sclerotiorum.In pot experiments,HB-10 strain exhibited a relative preventive efficacy of 69.42% against wheat sheath blight.Orthogonal tests and response surface analysis revealed the optimal growth medium for HB-10 strain consisted of 10 g/L glucose,20 g/L yeast extract,and 10 g/L KCl.The optimal fermentation conditions included a rotational speed of 215 r/min,temperature of 37℃,inoculum size of 4%,initial pH of 7.3,and a culture time of 84 h.After optimization,the average OD600 value of HB-10 strain at 24 h increased by 181.8%(5.92) compared to the non-optimized condition,resulting in improved inhibition rate against the pathogenic bacteria.These research findings provide a theoretical basis for the development and application of a biocontrol agent using Pseudomonas aeruginosa HB-10,in the prevention and control of wheat sheath blight.

Key words: wheat sheath blight, biocontrol bacterium, fermentation optimization, Pseudomonas aeruginosa

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