Synergistic Effect of a Biocontrol Bacterium D25 and Cyprodinil·Fludioxonil on the Control of Tomato Gray Mold

doi:10.16409/j.cnki.2095-039x.2022.04.018

Abstract

Abstract: In order to screen the fungicide complex composed of biocontrol bacterium D25 and chemical fungicide to improve the biocontrol efficacy on tomato gray mold as well as reducing the dosage of chemical fungicides,toxicity of 12 fungicides against Botrytis cinerea was firstly tested in vitro by toxic medium method Bio-compatibility between fungicides with strong antifungal activity and biocontrol bacterium D25 was determined by plate counting method.O ptimal ratio of fungicides and biocontrol bacteriun D25 was screened by the toxicity ratio,and control efficacy of the complex in suppressing tomato gray mold was further tested in pot experiment.The results showed that among 12 fungicides,Bacillus subtilis and Trichoderma harzianum had the strongest toxicity against B.cinerea,with EC₅₀ values of 4.564×10^-5 mg/L and 0.021 mg/L,respectively.Cyprodinil·fludioxonil had better toxicity,with EC₅₀ value of 0.043 mg/L.Two botanical fungicides,carvacrol and matrine·osthol,had the lowest toxicity,with EC₅₀ values of 12.469 mg/L and 19.220 mg/L,respectively.Biocompatibility test showed that no negative effect was found between cyprodinil·fludioxonil and bio-control bacterium D25.The complex of cyprodinil-fludioxonil (0.043 mg/L) and bio-control bacterium D25(7×10⁴CFU/mL) at a volume ratio of 5:5 performed synergistic effect,with the toxicity ratio of 1.3943.In the pot experiment,the control efficacy of the prevention group reached 72.29%,which was higher than that of the individual fungicide by 19.26% and 29.29%,respectively,while the control efficacy of the treatment group reached 61.57%,which was higher than that of the individual fungicide by 15.40% and 21.23%,respectively.In conclusion,combined application of bio-control bacterium D25 and cyprodinil·fludioxonil had a synergistic effect against B.cinerea,which reduced the application amount of cyprodinil·fludioxonil by 50% without reducing the control efficacy on tomato gray mold,and had great potential to be applied in field.

Key words: biocontrol bacterium, Botrytis cinerea, combined virulence, synergistic effect

CLC Number:

HUANG Xin, ZHENG Lining, GU Xuehu, XU Xuanwei, ZHANG Hao, WANG Yingchun. Synergistic Effect of a Biocontrol Bacterium D25 and Cyprodinil·Fludioxonil on the Control of Tomato Gray Mold[J]. Chinese Journal of Biological Control, 2022, 38(4): 860-867.

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