Application of Trichoderma Bioactive Metabolites-based Composite Formulation in Controlling of Rice Pests and Diseases

doi:10.16409/j.cnki.2095-039x.2025.02.055

Abstract

Abstract: To develop Trichoderma bioactive metabolites –based product for green pest control in rice, the shelf life of the bioactive metabolites was first evaluated. The results showed that after one year of storage at room temperature (25℃), the retention rate of its main components ranged between 86.76% and 99.24%, demonstrating excellent stability of the Trichoderma bioactive metabolites. And further, the synergistic effect of mixed use of Trichoderma bioactive metabolites–based composite formulation Wotaibao (prepared with Trichoderma culture filtrate, amino acid and mcicroelements) and different pesticides (in the reduced and full use) on the control of rice pest insects and fungal diseases was clarified through the two-year demonstration field trial in Shanghai areas. Field demonstration trials in Songjiang District showed that the effectiveness of Trichoderma bioactive metabolites–based composite formulation + 75% tebuconazole-pyrimethanil aqueous dispersible granule + selenium and silica fertilizer in preventing rice grain black spot (caused by Curvularia plantarum), rice spikelet rot (caused by Fusarium fujikuroi) and rice sheath blight (caused by Rhizoctonia solani) reached 54.31%, 35.54% and 70.15%, respectively, and the combined use significantly increased the Aldehydes flavor substances in rice grains, which were commonly considered as pivotal substances in Chinese Fragrant Rice; the prevention effect of Trichoderma bioactive metabolites–based composite formulation + 75% oxime-pentaconazole aqueous dispersible granule + selenium and silicon fertilizer against Rice Sheath Blight reached more than 77.91%, which was more than that of single use of two chemical pesticides. Field demonstration trials in Jinshan District showed that Trichoderma bioactive metabolites–based composite formulation mixed with polyhedrosis virus suspension of 3 billion PIB/mL, 50% pymetrozine and 23% kresoxim-methyl·epoxiconazole achieved control of 96.78% and 88.26% against the Rice Leaf Rollers (Cnaphalocrocis medinalis) and Rice Planthoppers (Nilaparvata lugens),respectively; however, a single spraying of the full amount of chemical pesticides was revealed only control of 55.73% and 44.54% against rice leaf rollers and rice planthoppers, respectively, obviously lower than the mixed use of Trichoderma active metabolites agent and chemical pesticides. Overall, the preparation of Trichoderma active metabolites agent held the advantages in the diversified active ingredients, long shelf-life, suitability, and significant synergistic effect with chemical pesticides for the prevention and control of rice pest insects and fungal diseases. This study provided a new technique for enhancing the green control effectiveness against rice pest insects and fungal diseases based on the combined use of Trichoderma active metabolites agent and low-dose pesticides. This study laid the foundation for further improving the quality of Trichoderma bioactive metabolites-based composite formulation and enhancing their application level in the green control of rice pests and diseases, thereby reducing the use of chemical pesticides.

Key words: Trichoderma bioactive metabolites, rice pest insects and fungal diseases, reduced use of pesticide, biological control, green prevention and control

CLC Number:

S476

BAI Zhenxu, JI Tiancen, CHEN Lusheng, ZHANG Weikang, CAO Yurong, ZHU Caihua, DING Chu, CHEN Yong, LU Mingchang, WANG Wenli, CHEN Jie. Application of Trichoderma Bioactive Metabolites-based Composite Formulation in Controlling of Rice Pests and Diseases[J]. Chinese Journal of Biological Control, 2025, 41(5): 1166-1178.

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